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Efficacy of the entomopathogenic fungi; Beauveria bassiana and Metarhizium anisopliae against the cowpea weevil, Callosobruchus maculatus F. (Coleoptera: Chrysomelidae: Bruchinae)
Egyptian Journal of Biological Pest Control ( IF 2.1 ) Pub Date : 2020-03-06 , DOI: 10.1186/s41938-020-00219-y
Ismail Oguz Ozdemir , Celal Tuncer , Ismail Erper , Rahman Kushiyev

The efficacy of the two entomopathogenic fungi, Metarhizium anisopliae (TR 106) and Beauveria bassiana (TR 217), was tested against the adults of the cowpea weevil, Callosobruchus maculatus F. (Coleoptera: Chrysomelidae: Bruchinae) in laboratory. Two concentrations of conidial suspensions (1 × 106 and 1 × 108 conidia/ml) of M. anisopliae and B. bassiana isolates were directly applied on four pairs of adult beetles in Petri dishes (2 ml/dish) and then incubated at two different climatic regimes; 22±1 °C and 26 ± 1 °C with 70 ± 5% RH, 16:8 h light-to-dark. Lethal time values (LT50 and LT90) were calculated using probit analysis. As a result, the LT50 and LT90 values of M. anisopliae (1 × 108 conidia/ml) were 4.45 and 5.34 days at 26 °C and 5.17 and 6.15 days at 22 °C, respectively. LT50 and LT90 values of B. bassiana (1 × 108 conidia/ml) were 4.07 and 5.11 days at 26 °C and 4.07 and 5.41 days at 22 °C, respectively. LT50 and LT90 values of M. anisopliae (1 × 106 conidia/ml) were 5.42 and 6.43 days at 26 °C and 6.08 and 7.54 days at 22 °C, respectively. The LT50 and LT90 values of B. bassiana (1 × 106 conidia/ml) were 5.67 and 7.15 days at 26 °C and 5.47 and 7.50 days at 22 °C, respectively. Approximately 100% of mycoses were obtained in all treatments. In general, the effectiveness of these two entomopathogens increased by increasing suspension concentrations and temperature. These results suggest that the two isolates may be very successful in biological control of the C. maculatus and may be alternatives for chemical pest management.

中文翻译:

昆虫病原真菌的功效;白僵菌绿僵菌对抗豇豆象甲,纹豆象F.(鞘翅目:叶甲科:Bruchinae)

在实验室中,对against豆象鼻虫(Callosobruchus maculatus F。)(鞘翅目:金眼科:Bruchinae)的成虫进行了测试,测试了两种昆虫病原真菌,Metrahizium anisopliae(TR 106)和球孢白僵菌(TR 217)。将两种浓度的分枝杆菌和球孢杆菌的分生孢子悬浮液(1×106和1×108分生孢子/ ml)直接应用于培养皿中的四对成年甲虫(2 ml /皿)中,然后在两个不同的温度下孵育气候状况;22±1°C和26±1°C,相对湿度70±5%,明暗时间为16:8 h。使用概率分析计算致死时间值(LT50和LT90)。结果,异链霉菌(1×108分生孢子/ ml)的LT50和LT90值在26°C下分别为4.45天和5.34天,在22°C下分别为5.17天和6.15天。球孢白僵菌的LT50和LT90值(1×108分生孢子/ ml)为4。在26°C下分别为07和5.11天,在22°C下分别为4.07和5.41天。沙门氏菌的LT50和LT90值(1×106分生孢子/ ml)在26°C下分别为5.42天和6.43天,在22°C下分别为6.08和7.54天。球孢杆菌(1×106分生孢子/ ml)的LT50和LT90值在26°C下分别为5.67天和7.15天,在22°C下分别为5.47天和7.50天。在所有处理中均获得了大约100%的真菌病。通常,这两种昆虫病原体的有效性通过增加悬浮液浓度和温度来提高。这些结果表明,这两种分离株在斑节梭菌的生物防治中可能非常成功,并且可能是化学病虫害防治的替代方法。在26°C下分别为43天,在22°C下分别为6.08和7.54天。球孢杆菌(1×106分生孢子/ ml)的LT50和LT90值在26°C下分别为5.67天和7.15天,在22°C下分别为5.47天和7.50天。在所有处理中均获得了大约100%的真菌病。通常,这两种昆虫病原体的有效性通过增加悬浮液浓度和温度来提高。这些结果表明,这两种分离株在斑节梭菌的生物防治中可能非常成功,并且可能是化学病虫害防治的替代方法。在26°C下分别为43天,在22°C下分别为6.08和7.54天。球孢杆菌(1×106分生孢子/ ml)的LT50和LT90值在26°C下分别为5.67天和7.15天,在22°C下分别为5.47天和7.50天。在所有处理中均获得了大约100%的真菌病。通常,这两种昆虫病原体的有效性通过增加悬浮液浓度和温度来提高。这些结果表明,这两种分离株在斑节对虾的生物学控制中可能非常成功,并且可能是化学害虫管理的替代方法。通过增加悬浮液的浓度和温度来提高这两种昆虫病原体的有效性。这些结果表明,这两种分离株在斑节梭菌的生物防治中可能非常成功,并且可能是化学病虫害防治的替代方法。通过增加悬浮液的浓度和温度来提高这两种昆虫病原体的有效性。这些结果表明,这两种分离株在斑节梭菌的生物防治中可能非常成功,并且可能是化学病虫害防治的替代方法。
更新日期:2020-03-06
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