Effects of freshwater sponge Ephydatia fluviatilis on conjugative transfer of antimicrobial resistance in Enterococcus faecalis strains in aquatic environments,Letters in Applied Microbiology

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Effects of freshwater sponge Ephydatia fluviatilis on conjugative transfer of antimicrobial resistance in Enterococcus faecalis strains in aquatic environments
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2020-06-04 , DOI: 10.1111/lam.13310
A Cartwright ₁ , J Arnscheidt ₁ , M Conwell ₂ , J S G Dooley ₂ , C McGonigle ₁ , P J Naughton ₂

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Filter feeding is a biotic process that brings waterborne bacteria in close contact with each other and may thus support the horizontal transfer of their antimicrobial resistance genes. This laboratory study investigated whether the freshwater sponge Ephydatia fluviatilis supported the transfer of vancomycin resistance between two Enterococcus faecalis strains that we previously demonstrated to exhibit pheromone responsive plasmid conjugation. Microcosm experiments exposed live and dead colonies of laboratory‐grown sponges to a vancomycin‐resistant donor strain and a rifampicin‐resistant recipient strain of Ent. faecalis. Enterococci with both resistance phenotypes were detected on double selection plates. In comparison to controls, abundance of these presumed transconjugants increased significantly in water from sponge microcosms. Homogenized suspensions of sponge cells also yielded presumed transconjugants; however, there was no significant difference between samples from live or dead sponges. Fluorescent in situ hybridization analysis of the sponge cell matrix using species‐specific probes revealed the presence of enterococci clusters with cells adjacent to each other. The results demonstrated that sponge colonies can support the horizontal transfer of antimicrobial resistance although the mechanism underlying this process, such as binding of the bacteria to the sponge collagen matrix, has yet to be fully elucidated.

中文翻译：

淡水海绵 Ephydatia fluviatilis 对水生环境中粪肠球菌菌株抗菌素耐药性结合转移的影响

滤食是一种生物过程，它使水生细菌彼此密切接触，从而可能支持其抗微生物基因的水平转移。该实验室研究调查了淡水海绵 Ephydatia fluviatilis 是否支持我们先前证明表现出信息素响应性质粒结合的两种粪肠球菌菌株之间万古霉素抗性的转移。微观世界实验将实验室培养海绵的活菌落和死菌落暴露于耐万古霉素的供体菌株和耐利福平的受体菌株。粪便。在双选择平板上检测到具有两种抗性表型的肠球菌。与对照相比，这些假定的转接合子的丰度在海绵微观世界的水中显着增加。海绵细胞的匀浆悬浮液也产生了推测的转接合子；然而，活海绵和死海绵的样本之间没有显着差异。使用物种特异性探针对海绵细胞基质进行的荧光原位杂交分析显示存在细胞彼此相邻的肠球菌簇。结果表明，海绵菌落可以支持抗菌素耐药性的水平转移，尽管这一过程的机制，例如细菌与海绵胶原蛋白基质的结合，尚未完全阐明。使用物种特异性探针对海绵细胞基质进行的荧光原位杂交分析显示存在细胞彼此相邻的肠球菌簇。结果表明，海绵菌落可以支持抗菌素耐药性的水平转移，尽管这一过程的机制，例如细菌与海绵胶原基质的结合，尚未完全阐明。使用物种特异性探针对海绵细胞基质进行的荧光原位杂交分析显示存在细胞彼此相邻的肠球菌簇。结果表明，海绵菌落可以支持抗菌素耐药性的水平转移，尽管这一过程的机制，例如细菌与海绵胶原基质的结合，尚未完全阐明。

更新日期：2020-06-04

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