LncRNA TUG1 has been rarely studied in ovarian cancer (OC), our objective was to explore the role of TUG1 in the regulation of malignant phenotypes of OC. Vectors of sh‐TUG1, miR‐186‐5p and pcDNA‐ZEB1 were, respectively, constructed and used to infect OC cells. MTT and transwell assays were applied for representing cell proliferation and invasion, respectively. Sphere formation experiment was used to detect the stemness of OC cells. Western blotting and qRT‐PCR were employed for detecting the expression of multiple biomarkers on protein and RNA levels, respectively. The luciferase assay was performed to reveal the interactions between miR‐186‐5p and TUG1 or ZEB1. The silencing of TUG1 and upregulation of miR‐186‐5p both suppressed the cell proliferation, invasion and cancer stem cell (CSC) properties. Additionally, luciferase assay verified that miR‐186‐5p directly binds TUG1 and ZEB1. Moreover, overexpression of ZEB1 rescued the impact on the proliferation, invasion and stemness of TUG1 silencing in OC. TUG1 sponges miR‐186‐5p to release ZEB1 and promotes the proliferation, invasion and stemness of OC cells, suggesting that TUG1 could be a potential therapeutic target for OC therapy.

中文翻译：

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LncRNA TUG1 通过 miR-186-5p/ZEB1 轴促进卵巢癌细胞的增殖、侵袭和干性。


LncRNA TUG1在卵巢癌（OC）中的研究很少，我们的目的是探讨TUG1在卵巢癌恶性表型调节中的作用。分别构建sh-TUG1、miR-186-5p和pcDNA-ZEB1载体并用于感染OC细胞。 MTT 和 Transwell 测定分别用于代表细胞增殖和侵袭。采用成球实验检测OC细胞的干性。 Western blotting和qRT-PCR分别用于检测多种生物标志物在蛋白质和RNA水平上的表达。进行荧光素酶测定以揭示 miR-186-5p 与 TUG1 或 ZEB1 之间的相互作用。 TUG1 的沉默和 miR-186-5p 的上调均抑制细胞增殖、侵袭和癌症干细胞 (CSC) 特性。此外，荧光素酶测定证实 miR-186-5p 直接结合 TUG1 和 ZEB1。此外，ZEB1的过度表达挽救了OC中TUG1沉默对增殖、侵袭和干性的影响。 TUG1 海绵 miR-186-5p 释放 ZEB1 并促进 OC 细胞的增殖、侵袭和干细胞性，表明 TUG1 可能是 OC 治疗的潜在治疗靶点。