Psychrotolerant species of the Bacillus cereus group, Bacillus mycoides and Bacillus weihenstephanensis, can grow at ≥ 7 °C and are significant concerns for the food industry due to their ability to cause spoilage of refrigerated food. In addition to that, some strains of B. weihenstephanensis can produce emetic toxin, namely cereulide, which is known to cause vomiting. Therefore, rapid and simple methods to discriminate psychrotolerant B. cereus group species are crucial. Here, matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) and the S10-spc-alpha operon gene encoded ribosomal protein mass spectrum (S10-GERMS) method were used to discriminate psychrotolerant species of the B. cereus group based on a set of four ribosomal subunit proteins (S10, S16, S20 and L30). A total of 36 strains of B. cereus group were cultured on LB agar, and analyzed by MALDI-TOF MS. The four biomarkers successfully discriminated 12 strains of psychrotolerant species from mesophilic species of the B. cereus group. Furthermore, the four biomarkers also classified some Bacillus thuringiensis strains.

MALDI-TOF MS analysis using the S10-GERMS method allowed simple and rapid discrimination of psychrotolerant species of the B. cereus group from other mesophilic species. This method has a possibility to enable manufacturers and distributors of refrigerated foods to control psychrotolerant species of the B. cereus group effectively.

在的Psychrotolerant种蜡样芽胞杆菌组，蕈状芽孢杆菌和weihenstephanensis芽孢杆菌，可在≥7生长℃，可以用于食品工业，由于其能力的冷藏食品变质引起关注显著。除此之外，维汉斯巴斯德杆菌的某些菌株还可以产生催吐毒素，即小肠内毒素，已知会引起呕吐。因此，快速，简单的方法来区分蜡状芽孢杆菌的物种。在这里，基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）和S10 - SPC - α操纵子基因编码的核糖体蛋白质谱（S10 -GERMS）方法用于基于一组四个核糖体亚基蛋白（S10，S16，S20和L30）来区分蜡状芽孢杆菌组的抗精神病物种。在LB琼脂上共培养蜡状芽孢杆菌组的36个菌株，并通过MALDI-TOF MS进行分析。四种生物标记物成功地从蜡状芽孢杆菌组的嗜温菌种中区分出了12种耐精神病菌种。此外，这四个生物标记物还对苏云金芽胞杆菌的一些菌株进行了分类。

使用S10 -GERMS方法进行的MALDI-TOF MS分析可以简单快速地将蜡状芽孢杆菌组的抗精神病物种与其他中温物种区分开。这种方法有可能使冷藏食品的制造商和分销商有效控制蜡状芽孢杆菌类的抗精神病物种。