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Hepato-nephrotoxicity in late juvenile of Oreochromis niloticus exposed to gibberellic acid: Ameliorative effect of Spirulina platensis
Pesticide Biochemistry and Physiology ( IF 4.2 ) Pub Date : 2020-05-01 , DOI: 10.1016/j.pestbp.2020.104600 Alaa El-Din H Sayed 1 , Elham A AbdAllah 2 , Mohamed Hamed 3 , Hamdy A M Soliman 4
Pesticide Biochemistry and Physiology ( IF 4.2 ) Pub Date : 2020-05-01 , DOI: 10.1016/j.pestbp.2020.104600 Alaa El-Din H Sayed 1 , Elham A AbdAllah 2 , Mohamed Hamed 3 , Hamdy A M Soliman 4
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The current work intended to inspect the hepato-nephrotoxicity of gibberellic acid (GA3) in juvenile of Oreochromis niloticus as well as the possibility of restoration after dietary addition of different concentrations of Spirulina platensis (SP). Fishes were evenly assorted into five groups: Group I assigned as control, Group II fed on basal diet and exposed to 150 mg/L gibberellic acid (GA3). The 3rd, 4th, and 5th groups exposed to150 mg/L gibberellic acid (GA3) and previously fed for two months on SP supplemented diets at levels of 5, 20, and 100 g/kg, respectively. Fish serum were utilized to check glucose, total protein, hepatic and renal functions, enzymatic and non-enzymatic antioxidants activities (superoxide dismautase; SOD, catalase; CAT, and total antioxidant capacity; TAC) as well as histopathological alterations in liver and kidney. The results showed that creatinine, uric acid, liver enzymes, glucose, total protein, SOD, and CAT were significantly elevated in GA3-treated group. Liver of GA3-treated fish manifested some histopathological changes (hypertrophy, cytoplasmic vacuolization, and apoptotic cells with pyknotic nuclei, necrosis, dilated blood sinusoids, and lymphocytic aggregation around the central veins). Kidney of GA3-exposed fish revealed edema of the epithelium lining of some renal tubules and some showed vacuolar degeneration and dissociation. Hypertrophy in the glomerulus was observed with dilated blood capillaries. SP supplementation restored these biochemical, antioxidants, and histological changes near to control levels. This improvement was higher with 100 g/kg SP showing concentration dependency. According to this study we can conclude that SP supplementation can improve the hepato- and nephrotoxicity caused by GA3 exposure indicating its role as potent antioxidant food additive.
中文翻译：
赤霉酸暴露对尼罗罗非鱼晚期幼鱼的肝肾毒性：螺旋藻的改善作用
目前的工作旨在检查赤霉酸（GA3）对尼罗罗非鱼幼鱼的肝肾毒性，以及在饲料中添加不同浓度的螺旋藻（SP）后恢复的可能性。鱼被均匀地分为五组：第一组作为对照组，第二组以基础饮食喂养并暴露于 150 mg/L 赤霉酸 (GA3)。第 3、第 4 和第 5 组暴露于 150 mg/L 赤霉酸 (GA3) 并且之前分别以 5、20 和 100 g/kg 的水平添加 SP 饮食两个月。鱼血清用于检查葡萄糖、总蛋白、肝肾功能、酶促和非酶促抗氧化活性（超氧化物歧化酶；SOD、过氧化氢酶；CAT 和总抗氧化能力；TAC）以及肝脏和肾脏的组织病理学改变。结果显示，GA3治疗组肌酐、尿酸、肝酶、葡萄糖、总蛋白、SOD和CAT显着升高。GA3 处理的鱼的肝脏表现出一些组织病理学变化（肥大、细胞质空泡化和具有固缩核的凋亡细胞、坏死、扩张的血窦和中央静脉周围的淋巴细胞聚集）。GA3 暴露鱼的肾脏显示一些肾小管的上皮衬里水肿，一些显示液泡变性和分离。在扩张的毛细血管中观察到肾小球肥大。补充 SP 使这些生化、抗氧化剂和组织学变化恢复到接近控制水平。当 100 g/kg SP 显示浓度依赖性时，这种改进更高。
更新日期：2020-05-01
中文翻译：
赤霉酸暴露对尼罗罗非鱼晚期幼鱼的肝肾毒性：螺旋藻的改善作用
目前的工作旨在检查赤霉酸（GA3）对尼罗罗非鱼幼鱼的肝肾毒性，以及在饲料中添加不同浓度的螺旋藻（SP）后恢复的可能性。鱼被均匀地分为五组：第一组作为对照组，第二组以基础饮食喂养并暴露于 150 mg/L 赤霉酸 (GA3)。第 3、第 4 和第 5 组暴露于 150 mg/L 赤霉酸 (GA3) 并且之前分别以 5、20 和 100 g/kg 的水平添加 SP 饮食两个月。鱼血清用于检查葡萄糖、总蛋白、肝肾功能、酶促和非酶促抗氧化活性（超氧化物歧化酶；SOD、过氧化氢酶；CAT 和总抗氧化能力；TAC）以及肝脏和肾脏的组织病理学改变。结果显示，GA3治疗组肌酐、尿酸、肝酶、葡萄糖、总蛋白、SOD和CAT显着升高。GA3 处理的鱼的肝脏表现出一些组织病理学变化（肥大、细胞质空泡化和具有固缩核的凋亡细胞、坏死、扩张的血窦和中央静脉周围的淋巴细胞聚集）。GA3 暴露鱼的肾脏显示一些肾小管的上皮衬里水肿，一些显示液泡变性和分离。在扩张的毛细血管中观察到肾小球肥大。补充 SP 使这些生化、抗氧化剂和组织学变化恢复到接近控制水平。当 100 g/kg SP 显示浓度依赖性时，这种改进更高。
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