Biosensors based on Organic Field-Effect Transistors (OFETs) have attracted increasing attention due to the possibility of rapid, label-free, and inexpensive detection. Among all the different possibilities, inkjet-printed top-gate organic Field Effect Transistors-Based Biosensors (BioFETs) using a polymeric gate insulator have been seldom reported. In this work, a systematic investigation in terms of topographical and electrical characterization was carried out in order to find the optimal fabrication process for obtaining a reliable polymer insulator. Previous studies have demonstrated that the best electrical performance arises from the use of the perfluoropolymer Cytop™[12,13,14]. Consequently, a simple immobilization protocol was used to ensure the proper attachment of a model biomolecule onto the Cytop's hydrophobic surface whilst keeping its remarkable insulating properties with gate current in the range of dozens of pico-amperes. The top-gate inkjet-printed BioFETs presented in this study operate at threshold voltages in the range of 1–2 V and show durability even when exposed to oxygen plasma, wet amine functionalization treatments, and aqueous media. As a preliminary application, the inkjet-printed top-gate BioFETs is used for monitoring an immunoreaction by measuring changes in the drain current, paving the way for further use of this device in the immunosensing field.

基于有机场效应晶体管（OFET）的生物传感器已经引起了越来越多的关注，这是因为它具有快速，无标记和廉价的检测可能性。在所有不同的可能性中，很少有报道使用聚合物栅绝缘体进行喷墨印刷的基于顶栅有机场效应晶体管的生物传感器（BioFET）。在这项工作中，进行了有关形貌和电气特性方面的系统研究，以便找到获得可靠的聚合物绝缘子的最佳制造工艺。先前的研究表明，使用全氟聚合物Cytop™[12,13,14]可获得最佳的电气性能。因此，使用了简单的固定方案以确保模型生物分子正确附着到Cytop' 疏水表面，同时保持其显着的绝缘性能，栅极电流范围为数十皮安。这项研究中介绍的顶栅喷墨印刷BioFET在1-2 V的阈值电压下工作，即使暴露于氧等离子体，湿胺官能化处理和水性介质中也显示出耐久性。作为初步应用，喷墨打印的顶栅BioFET用于通过测量漏极电流的变化来监视免疫反应，为在免疫传感领域进一步使用该设备铺平了道路。湿胺官能化处理和水性介质。作为初步应用，喷墨打印的顶栅BioFET用于通过测量漏极电流的变化来监视免疫反应，为在免疫传感领域进一步使用该设备铺平了道路。湿胺官能化处理和水性介质。作为初步应用，喷墨打印的顶栅BioFET用于通过测量漏极电流的变化来监视免疫反应，为在免疫传感领域进一步使用该设备铺平了道路。