SARS Coronavirus-2 (SARS-CoV-2) pandemic has become a global issue which has raised the concern of scientific community to design and discover a counter-measure against this deadly virus. So far, the pandemic has caused the death of hundreds of thousands of people upon infection and spreading. To date, no effective vaccine is available which can combat the infection caused by this virus. Therefore, this study was conducted to design possible epitope-based subunit vaccines against the SARS-CoV-2 virus using the approaches of reverse vaccinology and immunoinformatics. Upon continual computational experimentation, three possible vaccine constructs were designed and one vaccine construct was selected as the best vaccine based on molecular docking study which is supposed to effectively act against the SARS-CoV-2. Thereafter, the molecular dynamics simulation and in silico codon adaptation experiments were carried out in order to check biological stability and find effective mass production strategy of the selected vaccine. This study should contribute to uphold the present efforts of the researches to secure a definitive preventative measure against this lethal disease.

SARS冠状病毒2（SARS-CoV-2）大流行已经成为一个全球性问题，引起了科学界的关注，以设计和发现针对这种致命病毒的对策。迄今为止，该大流行病已导致数十万人在感染和扩散后死亡。迄今为止，还没有有效的疫苗可以抵抗这种病毒引起的感染。因此，本研究旨在利用反向疫苗学和免疫信息学方法设计针对SARS-CoV-2病毒的可能基于表位的亚单位疫苗。经过不断的计算实验，基于分子对接研究，设计了三种可能的疫苗构建体，并选择了一种疫苗构建体作为最佳疫苗，该疫苗应能有效对抗SARS-CoV-2。之后，为了验证生物学稳定性并找到所选疫苗的有效批量生产策略，进行了计算机密码子适应实验。这项研究应有助于坚持目前的研究努力，以确保针对这种致命疾病采取明确的预防措施。