Sodium azide is a commonly used cytochrome oxidase inhibitor that leads to translation repression and RNA granule assembly. The global changes in mRNA abundance in response to this stressor are unknown. RGG-motif proteins Scd6 and Sbp1 are translation-repressors and decapping-activators that localize to and affect the assembly of RNA granules in response to sodium azide stress. Transcriptome-wide effects of these proteins remain unknown. To address this we have sequenced transcriptome of the: a) wild type strain under unstressed and following sodium azide stress, b) Δscd6 and Δsbp1 strains under unstressed and following sodium azide stress. Transcriptome analysis identified altered abundance of many transcripts belonging to stress-responsive pathways validated by qRT-PCR results. Abundance of several transcripts was altered in Δscd6/Δsbp1 under normal conditions and upon stress. Overall, this study provides critical insights into transcriptome changes to sodium azide stress and the role of RGG-motif proteins in these changes.

中文翻译：

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通过转录组分析阐明 RNA 颗粒诱导叠氮化钠应激反应。


叠氮化钠是一种常用的细胞色素氧化酶抑制剂，可导致翻译抑制和 RNA 颗粒组装。响应这种应激源的 mRNA 丰度的整体变化尚不清楚。 RGG 基序蛋白 Scd6 和 Sbp1 是翻译抑制蛋白和脱帽激活蛋白，它们定位于并影响叠氮化钠应激反应中的 RNA 颗粒的组装。这些蛋白质的转录组范围的影响仍然未知。为了解决这个问题，我们对以下菌株的转录组进行了测序：a) 无应激和叠氮化钠应激后的野生型菌株，b) 无应激和叠氮化钠应激后的 Δscd6 和 Δsbp1 菌株。转录组分析发现，通过 qRT-PCR 结果验证，属于应激反应途径的许多转录本的丰度发生了变化。在正常条件下和应激条件下，Δscd6/Δsbp1 中几个转录本的丰度发生了变化。总的来说，这项研究为叠氮化钠应激导致的转录组变化以及 RGG 基序蛋白在这些变化中的作用提供了重要的见解。