Multiple infections by HPV genotypes are frequently detected in HPV+ cervical lesions but the interaction between each viral genotype during carcinogenesis is poorly understood. Here we carried out a comprehensive study to characterize the multiple HPV expression and integration by RNA-seq analyses of 19 invasive cervical carcinomas coinfected by several HPV genotypes. Analysis of tumor DNA by a hybridization assay indicated multiple infections ranging from 2 to 6 different HPV genotypes. RNA-seq analysis showed that a single HPV genotype was preferentially expressed. Finally, the search for HPV/human chimeric transcripts indicated integration from preferentially expressed genotypes. In conclusion, the present study indicated that, in invasive cervical carcinomas infected by multiple HPV genotypes, one HPV was preferentially expressed, supporting the hypothesis that a single HPV genotype was associated with cancer development.

中文翻译：

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HPV 基因型在被多种基因型感染的浸润性宫颈癌中的优先表达。

在 HPV+ 宫颈病变中经常检测到 HPV 基因型的多重感染，但对致癌过程中每种病毒基因型之间的相互作用知之甚少。在这里，我们进行了一项综合研究，通过 RNA-seq 分析对由多种 HPV 基因型共同感染的 19 种浸润性宫颈癌进行表征，以表征多种 HPV 表达和整合。通过杂交测定对肿瘤 DNA 的分析表明存在 2 到 6 种不同 HPV 基因型的多重感染。RNA-seq 分析表明，单一的 HPV 基因型优先表达。最后，对 HPV/人类嵌合转录物的搜索表明优先表达的基因型的整合。总之，本研究表明，在被多种HPV基因型感染的浸润性宫颈癌中，一种HPV优先表达，