The valorisation of Anaerobic digestion waste streams into algal biomass to produce a protein alternative to soybeans could have significant commercial and environmental value. It has the benefit of alleviating the pressure of disposal of nutrient-rich digestate that is rich in nitrogen, phosphorous and trace metals, while potentially reducing the cost of microalgae production. Currently, the use of soybean protein in animal feed has significant negative environmental issues and high carbon footprint associated with its use. This study investigates three types of Anaerobic digestion to grow Phaeodactylum tricornutum Bohlin microalgae. The results found that the crude protein in all concentrations of cow waste and food waste digestates were found to produce a significantly higher concentration of crude protein in comparison to the F/2 control. In addition, cow waste 1% and pig waste 1% formulations were found to have favourable fatty acid profiles, which has significant health benefits in the livestock industry. There was no significant difference in the total fatty acids found in cow waste 1% and pig waste 1% digestates compared to the F/2 control, which was in the range of 243.4 to 269.4 mg/g (dry weight). The other formulations produced a significantly lower (p < .05) concentration of total fatty acids compared to the control. Cow waste 1% was found to be richer in omega-3, eicosapentaenoic acid, compared to pig waste 1%, however, no significant difference was found between the eicosapentaenoic acid, concentration of cow waste 1% and the F/2 control. Overall, in terms of highest total fatty acids and crude protein, cow waste 1% digestate was found to perform the best out of all the digestates tested, and outperformed the F/2 control in terms of crude protein. The P. tricornutum grown in digestate was also found to bioaccumulate higher levels of calcium. P. tricornutum grown in cow waste 1% digestate could offer an alternative viable locally grown protein source for the animal feed industry, with the added advantage of being rich in eicosapentaenoic acid and calcium.

将厌氧消化废物流转化为藻类生物质以产生大豆蛋白的替代品可能具有重要的商业和环境价值。它的好处是减轻了处置富含氮，磷和微量金属的营养丰富的消化物的压力，同时有可能降低微藻类生产的成本。当前，在动物饲料中使用大豆蛋白具有严重的负面环境问题，并且与其使用相关的碳足迹高。这项研究调查了三种类型的厌氧消化法生长三角藻Bohlin微藻。结果发现，与F / 2对照相比，发现所有浓度的牛粪和食物残渣消化物中的粗蛋白均产生明显更高的粗蛋白浓度。此外，发现1％的牛废料和1％的猪废料具有良好的脂肪酸特性，在畜牧业中具有显着的健康益处。与F / 2对照相比，在1％的牛废料和1％的猪废料消化物中发现的总脂肪酸没有显着差异，其范围为243.4至269.4 mg / g（干重）。其他公式产生的值显着降低（p  <  .05）与对照组相比，总脂肪酸的浓度。与1％的猪粪相比，发现1％的猪粪富含omega-3二十碳五烯酸，但是，二十碳五烯酸，1％的牛粪废物浓度和F / 2对照之间没有发现显着差异。总体而言，就最高的总脂肪酸和粗蛋白而言，发现所有测试的消化物中，牛粪中1％的消化物表现最佳，就粗蛋白而言，其表现优于F / 2对照。该三角褐指藻的生长消化液还发现，生物体内积累更高水平的钙。三角果 在1％的消化液中生长的牛粪便可以为动物饲料工业提供另一种可行的本地生长的蛋白质来源，其另一个优点是富含二十碳五烯酸和钙。