Integrated systematics of Anopheles subpictus (Diptera: Culicidae) in the Oriental Region, with emphasis on forms in Thailand and Sulawesi, Indonesia.,Acta Tropica

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Integrated systematics of Anopheles subpictus (Diptera: Culicidae) in the Oriental Region, with emphasis on forms in Thailand and Sulawesi, Indonesia.
Acta Tropica ( IF 2.1 ) Pub Date : 2020-05-11 , DOI: 10.1016/j.actatropica.2020.105503
Parinya Wilai ₁ , Rusdiyah Sudirman Made Ali ₂ , Jassada Saingamsook ₁ , Atiporn Saeung ₁ , Anuluck Junkum ₁ , Catherine Walton ₃ , Ralph E Harbach ₄ , Pradya Somboon ₁

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The Anopheles subpictus complex consists of four species informally designated, based on fixed inversions of polytene chromosomes and morphology, as species A, B, C and D in India. However, recent studies revealed the presence of only species A and B in Sri Lanka. Little is known about the specific identity of the taxon in other countries in Asia. This paper reports the results of a molecular and morphological study of An. subpictus in Thailand and South Sulawesi, Indonesia. The maxillary palpi of most females from Thailand have the apical pale band longer than the subapical dark band, seta 7-I of pupae branched and short, and eggs with 18-25 float ridges. These characters do not agree with those described for species A, B, C and D in India. The females of An. subpictus from South Sulawesi usually have the subapical dark band of the maxillary palpus equal in length to the apical pale band. Phylogenetic analyses of sequences of the internal transcribed spacer 2 (ITS2) region of rDNA and the cytochrome c oxidase subunit I (COI) gene of mtDNA of specimens from Thailand, and South Sulawesi, and from various localities in GenBank, were conducted. ITS2 sequences of specimens from all localities in Thailand were identical, except for a small divergence in specimen from Phang Nga Province. Three distinct COI clades were detected in specimens from Chiang Mai Province in northern Thailand. However, crossing experiments between the three clades revealed no genetic incompatibility, suggesting that they were conspecific. ITS2 and COI sequences of most specimens from Thailand fell in clades other than those of An. subpictus species A and B and An. subpictus from Indonesia (East Nusa Tenggara, Java, South Sulawesi) and the Philippines. ITS2 sequences from South Sulawesi and East Nusa Tenggara were very similar, and fell in a clade consisting of specimen from Phang Nga in southern Thailand and sequences of some specimens from Cambodia and Vietnam, but their COI sequences were distinct. DNA sequences and morphological differences suggest the presence of two species within An. subpictus in Thailand, and more than one species in Indonesia.

中文翻译：

东方地区亚按蚊（双翅目：蚊科）的综合系统学，重点关注泰国和印度尼西亚苏拉威西岛的形态。

亚纹按蚊复合体由四个物种组成，根据多线染色体和形态的固定倒位，非正式地指定为印度的物种 A、B、C 和 D。然而，最近的研究表明斯里兰卡仅存在物种 A 和 B。对于亚洲其他国家该分类单元的具体身份知之甚少。本文报告了 An 的分子和形态学研究结果。 subpictus 分布于泰国和印度尼西亚南苏拉威西岛。泰国大多数雌性上颌须的顶端淡色带长于顶端下深色带，蛹的刚毛7-I分枝且短，卵有18-25条浮脊。这些特征与印度 A、B、C 和 D 种的描述不一致。安的雌性。来自南苏拉威西岛的亚画象通常上颌须的根尖下深色带的长度与根尖浅带的长度相等。对来自泰国和南苏拉威西以及 GenBank 中不同地点的标本的 rDNA 内转录间隔区 2 (ITS2) 区域和 mtDNA 细胞色素 c 氧化酶亚基 I (COI) 基因的序列进行系统发育分析。泰国各地标本的 ITS2 序列是相同的，除了攀牙省的标本略有差异。在泰国北部清迈省的标本中检测到了三个不同的 COI 分支。然而，这三个分支之间的杂交实验没有发现遗传不相容性，这表明它们是同种的。来自泰国的大多数标本的 ITS2 和 COI 序列属于除 An 之外的进化枝。 subpictus 种 A、B 和 An。 subpictus 来自印度尼西亚（东努沙登加拉、爪哇、南苏拉威西）和菲律宾。来自南苏拉威西岛和东努沙登加拉省的ITS2序列非常相似，属于由来自泰国南部攀牙府的标本以及来自柬埔寨和越南的一些标本的序列组成的进化枝，但它们的COI序列不同。 DNA序列和形态差异表明An中存在两个物种。泰国有 subpictus，印度尼西亚有不止一种。

更新日期：2020-05-11

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