The Anopheles subpictus complex consists of four species informally designated, based on fixed inversions of polytene chromosomes and morphology, as species A, B, C and D in India. However, recent studies revealed the presence of only species A and B in Sri Lanka. Little is known about the specific identity of the taxon in other countries in Asia. This paper reports the results of a molecular and morphological study of An. subpictus in Thailand and South Sulawesi, Indonesia. The maxillary palpi of most females from Thailand have the apical pale band longer than the subapical dark band, seta 7-I of pupae branched and short, and eggs with 18-25 float ridges. These characters do not agree with those described for species A, B, C and D in India. The females of An. subpictus from South Sulawesi usually have the subapical dark band of the maxillary palpus equal in length to the apical pale band. Phylogenetic analyses of sequences of the internal transcribed spacer 2 (ITS2) region of rDNA and the cytochrome c oxidase subunit I (COI) gene of mtDNA of specimens from Thailand, and South Sulawesi, and from various localities in GenBank, were conducted. ITS2 sequences of specimens from all localities in Thailand were identical, except for a small divergence in specimen from Phang Nga Province. Three distinct COI clades were detected in specimens from Chiang Mai Province in northern Thailand. However, crossing experiments between the three clades revealed no genetic incompatibility, suggesting that they were conspecific. ITS2 and COI sequences of most specimens from Thailand fell in clades other than those of An. subpictus species A and B and An. subpictus from Indonesia (East Nusa Tenggara, Java, South Sulawesi) and the Philippines. ITS2 sequences from South Sulawesi and East Nusa Tenggara were very similar, and fell in a clade consisting of specimen from Phang Nga in southern Thailand and sequences of some specimens from Cambodia and Vietnam, but their COI sequences were distinct. DNA sequences and morphological differences suggest the presence of two species within An. subpictus in Thailand, and more than one species in Indonesia.

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东方地区的按蚊（Diptera：Culicidae）的综合系统，重点是泰国和印度尼西亚苏拉威西岛的形式。

根据多角体染色体的固定倒置和形态，非正式按蚊亚种由四个物种组成，分别是印度的A，B，C和D。但是，最近的研究表明，斯里兰卡仅存在A和B物种。对于亚洲其他国家/地区的分类单元的具体身份知之甚少。本文报道了An的分子和形态学研究结果。泰国和印度尼西亚南苏拉威西岛的亚种。来自泰国的大多数雌性的上颚palpi的根尖淡带长于根尖下的暗带，set的seta 7-I分支且短，卵有18-25个浮脊。这些字符与印度有关物种A，B，C和D的描述不同。安的女性。来自南苏拉威西岛的亚种通常具有上颌骨的亚尖部暗带，其长度等于根部浅带。进行了rDNA的内部转录间隔区2（ITS2）区域序列和来自泰国，南苏拉威西岛以及GenBank各个地方的标本的mtDNA的细胞色素c氧化酶亚基I（COI）基因序列的系统进化分析。来自泰国各地的标本的ITS2序列相同，只是攀牙省的标本差异很小。在泰国北部清迈省的标本中检测到三个不同的COI进化枝。然而，三个进化枝之间的交叉实验显示没有遗传不相容性，表明它们是同种的。来自泰国的大多数标本的ITS2和COI序列均落在An以外的进化枝中。亚种A，B和An。印度尼西亚（东努沙登加拉，爪哇，南苏拉威西岛）和菲律宾的亚种。来自南苏拉威西岛和东努沙登加拉的ITS2序列非常相似，并落入由泰国南部攀牙（Phang Nga）标本和一些柬埔寨和越南标本组成的进化枝中，但它们的COI序列却截然不同。DNA序列和形态学差异表明An内有两个物种。在泰国的亚种，在印度尼西亚的一种以上。跌落在一个由泰国南部攀牙（Phang Nga）标本和一些柬埔寨和越南标本的序列组成的进化枝中，但它们的COI序列却很不同。DNA序列和形态学差异表明An内有两个物种。在泰国的亚种，在印度尼西亚的一种以上。跌落在一个由泰国南部攀牙（Phang Nga）标本和一些柬埔寨和越南标本的序列组成的进化枝中，但它们的COI序列却截然不同。DNA序列和形态学差异表明An内有两个物种。在泰国的亚种，在印度尼西亚的一种以上。