当前位置: X-MOL 学术Anim. Reprod. Sci. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Consequences of electromagnetic field (EMF) radiation during early pregnancy - androgen synthesis and release from the myometrium of pigs in vitro.
Animal Reproduction Science ( IF 2.2 ) Pub Date : 2020-05-11 , DOI: 10.1016/j.anireprosci.2020.106465
Anita Franczak 1 , Ewa M Waszkiewicz 1 , Wiktoria Kozlowska 1 , Agata Zmijewska 1 , Anna Koziorowska 2
Affiliation  

An electromagnetic field (EMF) has been found to affect reproductive processes in females. The aim of this study was to determine the effect of low, non-ionizing EMF radiation on the steroidogenic activity of myometrium collected from pigs during the fetal peri-implantation period. Myometrial slices were treated with an EMF (50 and 120 Hz, 2 and 4 h of incubation) and examined for the aromatase cytochrome P450 17α-hydroxylase/C17-20lyase (CYP17A1) and 3β-hydroxysteroid dehydrogenase/Δ5-Δ4 isomerase (HSD3B1) mRNA transcript abundance, cytochrome P450c17 and 3βHSD protein abundance and the secretion of androstenedione (A4) and testosterone (T). To determine whether progesterone (P4) functions as a protectant from EMF radiation, the selected slices were treated with P4. In slices incubated without P4, EMF at 50 Hz altered cytochrome P450c17 protein abundance (4 h), HSD3B1 mRNA transcript abundance (4 h) and A4 release (2 h) as well as T release (2 h) in P4-treated slices. The EMF at 120 Hz in non P4-treated slices altered A4 release (2 and 4 h) whereas in P4-treated slices altered CYP17A1 mRNA transcript abundance (4 h), 3βHSD protein abundance (4 h), A4 (4 h) and T release (2 h). In conclusion, EMF radiation in the myometrium collected during the peri-implantation period alters the CYP17A1 and HSD3B1 mRNA transcript and encoded protein abundance, and androgen release due to the time of treatment and P4 presence or absence. The P4 did not function directly as an obvious protector against EMF radiation in the myometrium of pigs during the fetal peri-implantation period.



中文翻译:

妊娠早期电磁场(EMF)辐射的后果-雄激素的合成以及体外猪的子宫肌层释放。

已发现电磁场(EMF)影响女性的生殖过程。这项研究的目的是确定低,非电离的EMF辐射对胎儿围着植入期间从猪收集的子宫肌层的类固醇生成活性的影响。肌层切片与EMF(温育50至120赫兹,2小时和4小时)处理,并检测所述芳香细胞色素P45017α羟化酶/ C17-20lyase(CYP17A1)和3 β羟基类固醇脱氢酶/Δ5-Δ4异构酶(HSD3B1)mRNA的转录物丰度,细胞色素P450C17和3 β HSD蛋白丰度和雄烯二酮的分泌(A 4)和睾酮(T)。确定是否黄体酮(P 4)起到防止EMF辐射的作用,将选定的切片用P 4处理。在没有P孵育切片4,EMF以50Hz改变细胞色素P450C17蛋白丰度(4小时),HSD3B1 mRNA的转录物丰度(4小时)和A 4中磷的释放(2小时),以及为T释放(2小时)4 -处理过的切片。以120Hz在非P中的EMF 4 -处理的切片,来改变4释放(2和4小时),而P中4 -处理的切片改变CYP17A1 mRNA的转录物丰度(4小时),3 β HSD蛋白丰度(4小时),A 4(4小时)和T释放(2小时)。综上所述,在植入前的子宫肌层中收集的EMF辐射会改变CYP17A1HSD3B1 mRNA的转录本和编码的蛋白丰度,以及由于治疗时间和P 4存在或不存在而引起的雄激素释放。P 4不能直接作为胎儿围着胚胎期子宫肌层中EMF辐射的明显保护剂。

更新日期:2020-05-11
down
wechat
bug