Purpose

The aim of this study was to evaluate the in vitro and in vivo efficacy of propolis against Ligictaluridus floridanus on Ictalurus punctatus gills.

Methods

This study used an ethanol extract of propolis at a concentration of 300 mg mL⁻¹, with a total polyphenol concentration of 2.77 mg mL⁻¹ as determined by chromatography. In vitro trial used propolis concentrations at 5, 30 as well as 60 mg L^–1 and a 70% ethanol control group to treat naturally infected catfish. The in vivo experiment was 16 weeks in duration, consisting of seventy-two fish infected by cohabitation and subjected to one-hour baths every 72 h during the trial.

Results

In vitro studies showed that parasites exposed to 60 mg L⁻¹ of propolis displayed a significant reduction (p < 0.05) in their survival time. In vivo studies confirmed the reduction of intensity and abundance of Ligictaluridus floridanus on the gills of channel catfish.

Conclusion

This study has provided the first challenge of propolis against L. floridanus on I. punctatus gills and shows that propolis has an anthelmintic effect.

中文翻译：

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蜂胶（蜂产品）对from鱼（Ictalurus punctatus）G的寄生虫（Ligictaluridus floridanus）的影响。

目的

这项研究的目的是评估蜂胶对马尾I（Ictalurus punctatus gill）上的Liligtaluridus floridanus的体外和体内功效。

方法

该研究使用蜂胶的乙醇提取物，其浓度为300 mg mL ^-1，通过色谱法测定的总多酚浓度为2.77 mg mL ^-1。体外试验使用了浓度为5、30、60 mg L ^–1的蜂胶和70％的乙醇对照组来治疗自然感染的cat鱼。体内实验时间为16周，由72条同居感染的鱼组成，并在试验期间每72小时接受一小时的沐浴。

结果

体外研究表明，暴露于60 mg L ^-1蜂胶的寄生虫 的存活时间显着降低（p <0.05）。体内研究证实，channel鱼Li的强度和数量减少。

结论

对这项研究提供了蜂胶的第一个挑战L. floridanus上一个毛虫鳃和显示，蜂胶具有驱虫效果。