Porcine Epidemic Diarrhea Virus (PEDV) causes severe diarrhea and mortality in piglets. Robust immunity may break the transmission cycle. Expression of antigens in maize grains is a promising method for producing low-cost vaccines. As a first step, we expressed maize constructs containing PEDV S1 spike protein targeted to various cellular locations including the cell wall, endoplasmic reticulum, and vacuole, and fused to carrier proteins E. coli heat labile subunit (LTB) and a dendritic cell (DC) binding peptide, and obtained sufficient antigen for oral immunization. Constructs targeting S1 to the ER or fused to carrier proteins produced high levels of antigen of greater than 20 mg/kg. Oral administration to pigs elicited serum neutralizing antibodies, supporting oral immunization as a practical and cost-effective PEDV vaccine.

中文翻译：

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玉米产生的猪流行性腹泻病毒刺突蛋白的口服递送在猪体内引发中和抗体。

猪流行性腹泻病毒 (PEDV) 导致仔猪严重腹泻和死亡。强大的抗扰性可能会破坏传输周期。在玉米粒中表达抗原是一种生产低成本疫苗的有前景的方法。作为第一步，我们表达了含有 PEDV S1 刺突蛋白的玉米构建体，该蛋白靶向不同的细胞位置，包括细胞壁、内质网和液泡，并融合到载体蛋白大肠杆菌热不稳定亚基 (LTB) 和树突状细胞 (DC )结合肽，并获得足够的抗原用于口服免疫。将 S1 靶向 ER 或与载体蛋白融合的构建体产生了高于 20 mg/kg 的高水平抗原。对猪进行口服给药会引发血清中和抗体，支持口服免疫作为一种实用且具有成本效益的 PEDV 疫苗。