2,5-Furandicarboxylic acid (FDCA), one of the top biomass-based platform chemical, is highly promising for resins and polymers, and it can be prepared from the bio-oxidation of hydroxymethyl furfural (HMF), which can be obtained mainly from lignocellulosic glucose that has a high production potential from not edible biomass.A native strain, Acinetobacter calcoaceticus NL14, that could convert HMF into FDCA is used for combining degradation and fermentation by consolidated bioprocessing (CBP). In this study, it was observed that the initial HMF concentration and pH neutralizer played important roles in the bioconversion of HMF, 5 g/L of HMF could be converted by 100% within 48 h with 0.5 g/L sodium carbonate (Na2CO3) with the production of 0.31 g/L FDCA. Extra glucose and hydrogen peroxide (H2O2) addition could further promote the production of FDCA to 0.54 g/L with 100% HMF conversion and a higher conversion rate. This report could provide a potential native bacterium for furan chemicals bioconversion and bioelimination, especially for FDCA bioproduction.

中文翻译：

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5-羟基羟甲基糠醛（HMF）通过天然专性好氧细菌钙乙酸不动杆菌NL14生物转化为2,5-呋喃二甲酸（FDCA）。

2,5-呋喃二甲酸（FDCA）是基于生物质的顶级平台化学品之一，对树脂和聚合物具有很高的前景，它可以通过羟甲基糠醛（HMF）的生物氧化来制备，主要可通过获得木质纤维素葡萄糖具有非食用生物质的高生产潜力。可以将HMF转化为FDCA的本地菌株Calcoaceticus NL14不动杆菌用于通过合并生物处理（CBP）结合降解和发酵。在这项研究中，观察到初始HMF浓度和pH中和剂在HMF的生物转化中起重要作用，用0.5 g / L碳酸钠（Na2CO3）在48小时内可以将5 g / L HMF转化为100％。产量为0.31 g / L FDCA。额外添加葡萄糖和过氧化氢（H2O2）可以进一步将FDCA的产量提高到0.54 g / L，HMF转化率为100％，转化率更高。该报告可为呋喃化学物质的生物转化和生物消除，尤其是FDCA生物生产提供潜在的天然细菌。