Our previous studies indicated that YIGSR-A99 peptides chemically conjugated to fibrin hydrogel (FH) and applied to wounded submandibular gland (SMG) in vivo, formed new organized salivary tissue, whereas wounded SMG treated with FH alone or in the absence of a scaffold showed disorganized collagen formation and poor tissue healing. While these studies indicated that damaged SMG grow and differentiate when treated with FH containing L1 peptide, they were performed only in female mice. However, there is a well-established sexual dimorphism present in mouse SMG (e.g., males develop well-differentiated granular convoluted tubules, but these structures are poorly developed in females) and little is known about how these sex differences influence wound healing events. Therefore, the goal of this study was to conduct comparative analyses of regeneration patterns in male and female mice using L1p-FH in a wounded SMG mouse model. Particularly, we focused on sex-dependent wound healing events such as macrophage polarization, vascularization, tissue organization, and collagen deposition, and how these events affect salivary gland functioning.

中文翻译：

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小鼠下颌腺的性别依赖性再生模式。

我们以前的研究表明，YIGSR-A99肽化学偶联至纤维蛋白水凝胶（FH）并在体内应用于下颌下腺（SMG），形成了新的有组织的唾液组织，而仅用FH或在没有支架的情况下治疗的SMG伤口胶原蛋白形成紊乱，组织愈合不良。这些研究表明，受损的SMG在用含L1肽的FH处理时会生长并分化，但仅在雌性小鼠中进行。但是，在小鼠SMG中存在公认的性二态性（例如，雄性会形成分化良好的颗粒状曲折小管，但雌性中这些结构的发育较差），而这些性别差异如何影响伤口愈合事件的知之甚少。因此，这项研究的目的是在受伤的SMG小鼠模型中使用L1p-FH对雄性和雌性小鼠的再生模式进行比较分析。特别是，我们专注于性别相关的伤口愈合事件，例如巨噬细胞极化，血管形成，组织组织和胶原蛋白沉积，以及这些事件如何影响唾液腺功能。