Fluorescence imaging is indispensable to biomedical research, and yet it remains challenging to image through dynamic scattering samples. Techniques that combine ultrasound and light as exemplified by ultrasound-assisted wavefront shaping have enabled fluorescence imaging through scattering media. However, the translation of these techniques into in vivo applications has been hindered by the lack of high-speed solutions to counter the fast speckle decorrelation of dynamic tissue. Here, we report an ultrasound-enabled optical imaging method that instead leverages the dynamic nature to perform imaging. The method utilizes the correlation between the dynamic speckle-encoded fluorescence and ultrasound-modulated light signal that originate from the same location within a sample. We image fluorescent targets with an improved resolution of ≤75 µm (versus a resolution of 1.3 mm with direct optical imaging) within a scattering medium with 17 ms decorrelation time. This new imaging modality paves the way for fluorescence imaging in highly scattering tissue in vivo.

荧光成像对于生物医学研究是必不可少的，但是通过动态散射样品成像仍然具有挑战性。以超声辅助波阵面成形为例的结合超声和光的技术已使通过散射介质的荧光成像成为可能。但是，由于缺乏解决动态组织快速散斑去相关的高速解决方案，阻碍了这些技术向体内应用的转化。在这里，我们报告了一种启用超声的光学成像方法，该方法利用了动态特性来执行成像。该方法利用了动态散斑编码的荧光和超声调制的光信号之间的相关性，这些信号源自样品中的相同位置。我们在17 ms的去相关时间下，在散射介质中以≤75 µm（相对于直接光学成像为1.3 mm的分辨率）改善的分辨率对荧光目标进行成像。这种新的成像方式为体内高度散射的组织中的荧光成像铺平了道路。