Glial cell line-derived neurotrophic factor (GDNF) was initially described as important for dopaminergic neuronal survival and is involved in many other essential functions in the central nervous system. Characterization of GDNF phenotype in mammals is well described; however, studies in non-mammalian vertebrate models are scarce. Here, we characterized the anatomical distribution of gdnf-expressing cells in adult zebrafish brain by means of combined in situ hybridization (ISH) and immunohistochemistry. Our results revealed that gdnf was widely dispersed in the brain. gdnf transcripts were co-localized with radial glial cells along the ventricular area of the telencephalon and in the hypothalamus. Interestingly, Sox2 positive cells expressed gdnf in the neuronal layer but not in the ventricular zone of the telencephalon. A subset of GABAergic precursor cells labeled with dlx6a-1.4kbdlx5a/6a: green fluorescence protein (GFP) in the pallium, parvocellular preoptic nucleus, and the anterior and dorsal zones of the periventricular hypothalamus also showed expression with gdnf mRNA. In addition, gdnf signals were detected in subsets of dopaminergic neurons, including those in the ventral diencephalon, similar to what is seen in mammalian brain. Our work extends our knowledge of gdnf action sites and suggests a potential role for gdnf in adult brain neurogenesis and regeneration.

中文翻译：

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gdnf在成年斑马鱼脑中的细胞定位。

胶质细胞源性神经营养因子（GDNF）最初被描述为对多巴胺能神经元存活很重要，并且参与中枢神经系统的许多其他基本功能。哺乳动物中GDNF表型的表征已得到很好的描述。但是，在非哺乳动物脊椎动物模型中的研究很少。在这里，我们通过结合原位杂交（ISH）和免疫组织化学的方法表征了成年斑马鱼脑中表达gdnf的细胞的解剖分布。我们的结果表明gdnf广泛分散在大脑中。gdnf成绩单与放射状神经胶质细胞共定位在沿脑的脑室区域和下丘脑。有趣的是，Sox2阳性细胞在神经元层表达gdnf，但在末梢脑的心室区不表达。用dlx6a-1.4kbdlx5a / 6a标记的GABA能前体细胞的一个子集：大脑皮层，小细胞前视神经核以及下丘脑下丘脑前区和背区的绿色荧光蛋白（GFP）也显示了gdnf mRNA的表达。此外，在多巴胺能神经元的子集（包括腹侧中脑神经元）中检测到gdnf信号，类似于在哺乳动物脑中所见。我们的工作扩展了我们对gdnf作用位点的了解，并暗示了gdnf在成人脑神经发生和再生中的潜在作用。在多巴胺能神经元的子集中（包括腹侧中脑的神经元）检测到gdnf信号，这与在哺乳动物脑中发现的信号类似。我们的工作扩展了我们对gdnf作用位点的了解，并暗示了gdnf在成人脑神经发生和再生中的潜在作用。gdnf信号在多巴胺能神经元的子集中被检测到，包括在腹侧中脑的神经元中，类似于在哺乳动物脑中发现的信号。我们的工作扩展了我们对gdnf作用位点的了解，并暗示了gdnf在成人脑神经发生和再生中的潜在作用。