Treatment of Helicobacter pylori (H. pylori) infection is a challenge for clinicians. The large increase in drug-resistant strains makes the formulation of new therapeutic strategies fundamental. The frequent onset of side effects during antibiotic treatment (mainly due to intestinal dysbiosis) should not be underestimated as it may cause the interruption of treatment, failure of H. pylori eradication and clonal selection of resistant bacteria. Probiotic integration during antibiotic treatment can exert a dual function: a direct antagonistic effect on H. pylori and a balancing effect on dysbiosis. Therefore, it fulfills the definition of a new therapeutic strategy to successfully treat H. pylori infection. Data reported in literature give promising but discrepant results. AIM To assess in vitro bacteriostatic and bactericidal activity of probiotic strains against H. pylori. MATERIALS AND METHODS L. casei, L. paracasei, L. acidophilus, B. lactis and S. thermophilus strains were used. Agar well diffusion and time-kill curves were carried out to detect bacteriostatic and bactericidal activity, respectively. RESULTS All probiotic strains showed both bacteriostatic and bactericidal activity vs. H. pylori. CONCLUSIONS Such findings prompted us to plan a protocol of treatment in which probiotics are given to infected patients in association with antibiotic therapy.

中文翻译：

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五个益生菌菌株对幽门螺杆菌的抗菌作用。

幽门螺杆菌（H. pylori）感染的治疗是临床医生面临的挑战。耐药菌株的大量增加使得制定新的治疗策略至关重要。不应低估抗生素治疗期间频繁发生的副作用（主要是由于肠道营养不良），因为它可能会导致治疗中断，幽门螺杆菌根除失败和耐药菌的克隆选择。抗生素治疗过程中的益生菌整合可以发挥双重作用：对幽门螺杆菌具有直接的拮抗作用，对营养不良有平衡作用。因此，它满足了成功治疗幽门螺杆菌感染的新治疗策略的定义。文献中报道的数据给出了有希望但不一致的结果。目的评估益生菌菌株对幽门螺杆菌的体外抑菌和杀菌活性。材料与方法使用干酪乳杆菌，副干酪乳杆菌，嗜酸乳杆菌，乳酸杆菌和嗜热链球菌菌株。进行琼脂井扩散和时间杀灭曲线，分别检测抑菌和杀菌活性。结果所有益生菌菌株均相对于幽门螺杆菌显示出抑菌和杀菌活性。结论这些发现促使我们制定了一种治疗方案，即结合抗生素治疗向受感染的患者提供益生菌。分别。结果所有益生菌菌株均相对于幽门螺杆菌显示出抑菌和杀菌活性。结论这些发现促使我们制定了一种治疗方案，即结合抗生素治疗向受感染的患者提供益生菌。分别。结果所有益生菌菌株均相对于幽门螺杆菌显示出抑菌和杀菌活性。结论这些发现促使我们制定了一种治疗方案，即结合抗生素治疗向受感染的患者提供益生菌。