当前位置：
X-MOL 学术
›
Biotechnol. Appl. Bioc.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
RuBisCO nano enzyme for mimicking CO2 conversion system in plants
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2020-05-10 , DOI: 10.1002/bab.1937 Özlem Biçen Ünlüer 1 , Rıdvan Say 2, 3 , Arzu Ersöz 1
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2020-05-10 , DOI: 10.1002/bab.1937 Özlem Biçen Ünlüer 1 , Rıdvan Say 2, 3 , Arzu Ersöz 1
Affiliation
|
In this study, carbon dioxide (CO2) capture and conversion systems based on the combination of biomimetic systems with nano enzymes have been developed. The effectiveness of the developed system has been investigated toward CO2 conversion. For this aim, nano ribulose bisphosphate carboxylase/oxygenase (RuBisCO) enzyme that plays role in the Calvin cycle in photosynthesis has been synthesized in 93 nm size according to AmiNoAcid (monomer) Decorated and Light Underpinning Conjugation Approach (ANADOLUCA) method. Enzymatic activity of synthesized nano RuBisCO enzyme has been spectrophotometrically determined by the formation of 3‐phosphoglycerate (3‐PGA) at the end of the reaction between CO2 and d‐ribulose‐1,5 biphosphate with the catalysis of RuBisCO enzyme at 340 nm. The effect of substrate concentration, pH, temperature, and Mg2+ ion concentration on the conversion reaction have investigated comparatively with nano and free RuBisCO enzyme. Besides this, the reusability feature of synthesized nano RuBisCO enzyme in conversion of CO2 reaction is indicated. When all data were evaluated, it has been seen that the nano RuBisCO enzyme is effective on the conversion of CO2 into 3‐PGA and can be used for CO2 capture and conversion systems repeatedly without any deformation in its structure.
中文翻译：
RuBisCO纳米酶，用于模拟植物中的CO2转化系统
在这项研究中，已经开发了基于仿生系统与纳米酶结合的二氧化碳（CO 2）捕获和转化系统。已经研究了开发的系统对CO 2转化的有效性。为此，根据AmiNoAcid（单体）修饰和轻质基础共轭方法（ANADOLUCA）的方法，合成了在光合作用中的加尔文循环中起作用的纳米核糖二磷酸羧化酶/加氧酶（RuBisCO）酶。合成的纳米RuBisCO酶的酶活性已通过分光光度法测定，即在CO 2和d之间的反应结束时形成了3-磷酸甘油酸酯（3-PGA）。RuBisCO酶在340 nm处催化核糖1,5,5磷酸二氢盐。用纳米和游离RuBisCO酶比较研究了底物浓度，pH，温度和Mg 2+离子浓度对转化反应的影响。除此之外，还表明了合成的纳米RuBisCO酶在CO 2反应转化中的可重用性。对所有数据进行评估后，我们发现纳米RuBisCO酶可有效地将CO 2转化为3-PGA，并可以重复用于CO 2捕获和转化系统，而不会发生结构变形。
更新日期：2020-05-10
中文翻译：
RuBisCO纳米酶，用于模拟植物中的CO2转化系统
在这项研究中，已经开发了基于仿生系统与纳米酶结合的二氧化碳（CO 2）捕获和转化系统。已经研究了开发的系统对CO 2转化的有效性。为此，根据AmiNoAcid（单体）修饰和轻质基础共轭方法（ANADOLUCA）的方法，合成了在光合作用中的加尔文循环中起作用的纳米核糖二磷酸羧化酶/加氧酶（RuBisCO）酶。合成的纳米RuBisCO酶的酶活性已通过分光光度法测定，即在CO 2和d之间的反应结束时形成了3-磷酸甘油酸酯（3-PGA）。RuBisCO酶在340 nm处催化核糖1,5,5磷酸二氢盐。用纳米和游离RuBisCO酶比较研究了底物浓度，pH，温度和Mg 2+离子浓度对转化反应的影响。除此之外，还表明了合成的纳米RuBisCO酶在CO 2反应转化中的可重用性。对所有数据进行评估后，我们发现纳米RuBisCO酶可有效地将CO 2转化为3-PGA，并可以重复用于CO 2捕获和转化系统，而不会发生结构变形。
京公网安备 11010802027423号