Two East Asian grape species Vitis amurensis and Vitis coignetiae have been investigated for their increased cold and drought tolerance and high level of resistance to infections. Nowadays, the grape species attract attention due to their capability to produce a group of valuable plant secondary metabolites named stilbenes. The basic stilbene, t-resveratrol, is known to demonstrate a high bioactivity and is synthesized by stilbene synthase (STS) in grape cells. In order to find the suitable biological model for t-resveratrol production, 12 cell culture lines derived from the leaf, petiole and stem explants of the East Asian grape species were tested for their ability to produce t-resveratrol and other stilbenes. The comparative analysis showed that the V. coignetiae cell culture lines were the most productive in terms of stilbene content. This observation was confirmed by the increased level of STS expression in V. coignetiae cells in comparison to V. amurensis. The expression analysis of the genes encoding transcription factors potentially involved in regulation of STS genes revealed the significant species-specific differences, suggesting the molecular basis underlying the observed differences in stilbene biosynthetic potential of V. amurensis and V. coignetiae.

已经对两种东亚葡萄品种Vitis amurensis和Vitis coignetiae的耐寒和干旱耐受性以及对感染的高抵抗力进行了研究。如今，葡萄品种因其能够产生一组有价值的植物次生代谢产物斯提尔本的能力而备受关注。已知基本的二苯乙烯-叔白藜芦醇具有很高的生物活性，是由二苯乙烯-合酶（STS）在葡萄细胞中合成的。为了找到对适合的生物模型吨-白藜芦醇生产，12个细胞培养系从叶，叶柄衍生和干东亚葡萄物种的外植体测试其能力以产生吨-白藜芦醇和其他丁苯橡胶。对比分析表明，就二苯乙烯含量而言，弓形虫细胞培养细胞系生产力最高。该观察结果通过的增加的水平证实STS中表达V. coignetiae细胞相比，山葡萄。编码可能参与调节转录因子的基因的表达分析STS基因揭示了显著物种特异性差异，这在底层的茋生物合成电位的观察到的差异的分子基础山葡萄和V. coignetiae。