Gut microorganisms play an important role in host health and have susceptibility to diseases, undergoing a distinct transformation at early stages of development. This study aimed to determine the regulation of gut microorganism development from fertilized eggs to 150 DAH (days after hatching) and the differences between flatulent and healthy silver pomfret (Pampus argenteus). High‐throughput sequencing was used to analyze the diversity and relative abundance of microorganisms. PICRUSt was used to predict functional alterations of dominant microorganisms. The results showed that from all 42 samples, a total of 3,746,895 reads were obtained, representing 6,081 operational taxonomic units (OTUs). Top 3 phyla were Proteobacteria, Firmicutes and Bacteroidetes. From 0 to 32 DAH, the relative abundance of Proteobacteria continuously increased and peaked at 94.99%. After that, the abundance of Proteobacteria decreased, but Bacteroidetes and Firmicutes increased instead. Alpha diversity is the lowest at 32 DAH, the difference is significant compared with others, p < .05. Comparing flatulent fish with healthy fish, the former has more specific OTUs, higher diversity and abundance. There were four genera Ethanoligenens, Methanobacterium, Caproiciproducens and Leptolinea that only obtained from the flatulent samples, but almost none in the healthy samples. All of these four genera are directly or indirectly involved in gas metabolism. This study enriched the basic research of silver pomfret, for the first time, focused on flatulent individuals which would provide a theoretical basis for the subsequent breeding.

中文翻译：

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po发生过程中肠道微生物多样性与flat鱼肠胃气胀的相关性

肠道微生物在宿主健康中起重要作用，对疾病易感，在发育的早期阶段经历了明显的转化。这项研究旨在确定从受精卵到孵卵后150 DAH（孵化后几天）的肠道微生物发育的调控，以及肠胀气和健康银po的差异（Pampus argenteus）。高通量测序用于分析微生物的多样性和相对丰度。PICRUSt被用来预测优势微生物的功能变化。结果显示，从所有42个样本中，总共获得了3,746,895个读数，代表6,081个操作生物分类单位（OTU）。前三个门是Proteobacteria，Firmicutes和Bacteroidetes。从0到32 DAH，变形杆菌的相对丰度持续增加，并达到94.99％的峰值。在那之后，Proteobacteria的丰度下降，而拟杆菌和Firmicutes却增加了。α多样性在32 DAH时最低，与其他相比差异显着，p <.05。将肠胃气胀的鱼与健康的鱼进行比较，前者具有更特定的OTU，更高的多样性和丰度。仅从肠胃气胀的样品中获得了四个属的嗜酸杆菌属，甲烷菌，产腐殖质和小球藻，而在健康样品中几乎没有。这四个属均直接或间接参与气体代谢。这项研究首次丰富了银po的基础研究，其重点是肠胃气胀的个体，这将为随后的繁殖提供理论基础。