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miR-324-5p promotes adipocyte differentiation and lipid droplet accumulation by targeting Krueppel-like factor 3 (KLF3).
Journal of Cellular Physiology ( IF 4.5 ) Pub Date : 2020-05-09 , DOI: 10.1002/jcp.29652 Xiaomin Zhou 1 , Xin'e Shi 1 , Jie Wang 1 , Xiaoyu Zhang 1 , Yanting Xu 1 , Yihao Liu 1 , Xiao Li 1 , Gongshe Yang 1
Journal of Cellular Physiology ( IF 4.5 ) Pub Date : 2020-05-09 , DOI: 10.1002/jcp.29652 Xiaomin Zhou 1 , Xin'e Shi 1 , Jie Wang 1 , Xiaoyu Zhang 1 , Yanting Xu 1 , Yihao Liu 1 , Xiao Li 1 , Gongshe Yang 1
Affiliation
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miRNAs, a kind of noncoding small RNA, play a significant role in adipose differentiation. In this study, we explored the effect of miR‐324‐5p in adipose differentiation, and found that miR‐324‐5p could promote adipocytes differentiation and increase body weight in mice. We overexpressed miR‐324‐5p during adipocytes differentiation, by oil red O and bodipy staining found that lipid accumulation was increased, and the expression level of adipogenic related genes were significantly increased. And the opposite experimental results were obtained after inhibiting miR‐324‐5p. In vivo, we injected miR‐324‐5p agomiR in obese mice and found that body weight, adipocyte area, and adipogenic‐related gene expression level were significantly increased but lipolytic genes were decreased. To further explore the mechanism of miR‐324‐5p regulation in lipid accumulation, we constructed Krueppel‐like factor 3 (KLF3 ) 3′‐untranslated region luciferase reporter vector and KLF3 pcDNA 3.1 overexpression vector, and found that miR‐324‐5p was able to directly target KLF3 . Overall, in this study we found that miR‐324‐5p could promote mice preadipoytes differentiation and increase mice fat accumulation by targeting KLF3 .
中文翻译：
miR-324-5p通过靶向Krueppel样因子3（KLF3）促进脂肪细胞分化和脂质滴积累。
miRNA是一种非编码小RNA，在脂肪分化中起着重要作用。在这项研究中，我们探索了miR-324-5p在脂肪分化中的作用，并发现miR-324-5p可以促进小鼠脂肪细胞分化并增加体重。我们通过脂肪红O和bodipy染色在脂肪细胞分化过程中过表达miR-324-5p，发现脂质蓄积增加，并且脂肪形成相关基因的表达水平显着增加。抑制miR-324-5p后获得相反的实验结果。在体内，我们向肥胖小鼠注射了miR-324-5p agomiR，发现体重，脂肪细胞面积和脂肪形成相关基因的表达水平显着增加，而脂肪分解基因则减少。KLF3）3'-非翻译区荧光素酶报告载体和KLF3 pcDNA 3.1过表达载体，发现miR-324-5p能够直接靶向KLF3。总的来说，在这项研究中，我们发现miR-324-5p可以通过靶向KLF3促进小鼠前脂肪细胞分化并增加小鼠脂肪的积累。
更新日期：2020-05-09
中文翻译：
miR-324-5p通过靶向Krueppel样因子3（KLF3）促进脂肪细胞分化和脂质滴积累。
miRNA是一种非编码小RNA，在脂肪分化中起着重要作用。在这项研究中，我们探索了miR-324-5p在脂肪分化中的作用，并发现miR-324-5p可以促进小鼠脂肪细胞分化并增加体重。我们通过脂肪红O和bodipy染色在脂肪细胞分化过程中过表达miR-324-5p，发现脂质蓄积增加，并且脂肪形成相关基因的表达水平显着增加。抑制miR-324-5p后获得相反的实验结果。在体内，我们向肥胖小鼠注射了miR-324-5p agomiR，发现体重，脂肪细胞面积和脂肪形成相关基因的表达水平显着增加，而脂肪分解基因则减少。KLF3）3'-非翻译区荧光素酶报告载体和KLF3 pcDNA 3.1过表达载体，发现miR-324-5p能够直接靶向KLF3。总的来说，在这项研究中，我们发现miR-324-5p可以通过靶向KLF3促进小鼠前脂肪细胞分化并增加小鼠脂肪的积累。
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