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Water stress mediated root trait dynamics and identification of microsatellite markers associated with root traits in rice (Oryza sativa L.).
Physiology and Molecular Biology of Plants ( IF 3.4 ) Pub Date : 2020-05-09 , DOI: 10.1007/s12298-020-00809-y
R Rejeth 1 , Ch L N Manikanta 1 , R Beena 1 , Roy Stephen 1 , R V Manju 1 , M M Viji 1
Affiliation  

To identify microsatellite markers associated with root traits for drought tolerance in rice (Oryza sativa L.) a study was conducted at Department of Plant Physiology, College of Agriculture, Trivandrum, Kerala Agricultural University. A set of thirty-five rice genotypes were exposed to water stress and evaluated for physio-morphological components as indices of water stress tolerance. Observations were made on leaf rolling score and root traits, especially the root length, root dry weight, root volume and root shoot ratio at booting stage. As of the data obtained, ten tolerant and ten susceptible varieties were selected for bulk line analysis to identify the DNA markers linked with target gene conferring drought tolerance. Out of 150 SSR primers screened, RM474 showed polymorphism between the tolerant and susceptible bulks. Individual genotypes of the bulks also showed the same product size of the respective tolerant and susceptible bulks.

中文翻译:

水分胁迫介导的根系性状动态和与水稻(Oryza sativa L.)根系性状相关的微卫星标记的鉴定。

识别与根系性状的水稻抗旱性相关的微卫星分子标记(水稻L.)在喀拉拉邦农业大学Trivandrum农业学院植物生理学系进行了一项研究。一组三十五个水稻基因型暴露于水分胁迫下,并评估了生理形态成分作为水分胁迫耐受性的指标。观察了孕穗期叶片滚动得分和根系性状,尤其是根长,根干重,根体积和根梢比。从获得的数据中,选择十个耐性品种和十个易感品种进行批量分析,以鉴定与赋予抗旱性的靶基因相关的DNA标记。在筛选的150份SSR引物中,RM474在耐性和易感性之间存在多态性。散装的个别基因型也显示出各自耐受和易感散装的相同产品尺寸。
更新日期:2020-05-09
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