The genetic diversity of 33 Paris polyphylla samples collected from the Dabie Mountains was analyzed using SCoT and SRAP molecular markers, revealing the genetic relationships among Paris polyphylla resources in the Dabie Mountains at the molecular level and providing a theoretical basis for genetic improvement and conservation. As a result, a total of 134 bands were amplified with 9 SCoT primers, the percentage of polymorphic bands was 100%, the average number of primers amplified was 14.89, the PIC value was 94.83% and the genetic similarity coefficient ranged from 0.463 to 0.896. Ten pairs of SRAP primer combinations amplified 135 bands, including 129 polymorphic bands, and the percentage of polymorphic bands was 95.56%. The average number of polymorphic bands obtained with each pair of SRAP primer combinations was 12.9, the PIC value was 93.91%, and the genetic similarity coefficient ranged from 0.533 to 0.904. This study showed that both SCoT and SRAP markers were suitable for the genetic diversity analysis of P. polyphylla, which belongs to a genus in which SRAP marker technology has not previously been applied, despite its application in a variety of other plants.

中文翻译：

---

使用SCoT和SRAP标记评估巴黎重瓣种群之间的遗传关系和多样性。

利用SCoT和SRAP分子标记分析了从大别山采集的33种巴黎多叶植物的遗传多样性，揭示了巴黎多叶植物之间的遗传关系。大别山的分子资源水平，为遗传改良和保护提供理论依据。结果用9个SCoT引物扩增了134条带，多态性条带百分比为100％，平均引物数为14.89，PIC值为94.83％，遗传相似系数为0.463至0.896 。十对SRAP引物组合扩增出135条带，包括129条多态性条带，多态性条带百分比为95.56％。每对SRAP引物组合获得的平均多态性条带数目为12.9，PIC值为93.91％，遗传相似系数为0.533至0.904。这项研究表明，SCoT和SRAP标记都适合于玉米的遗传多样性分析。P. polyphylla，属于一个属，尽管它已在多种其他植物中应用，但以前尚未应用SRAP标记技术。