We have developed an analytical method based on pressurised liquid extraction (PLE) followed by liquid chromatography coupled to high resolution mass spectrometry (LC-HRMS) for the simultaneous determination of five phthalate diesters and six phthalate monoesters in widely consumed seafood species. In order to obtain the highest extraction recoveries, as well as to reduce the matrix effect of such complex samples, the most important extraction parameters were optimised and several clean-up and preconcentration strategies were tested. This method provided limits of detection that ranged from 1 to 25 ng g⁻¹ dry weight (d.w.) for most compounds in both fatty and low-fat species. Moreover, several target analytes were quantified in five seafood species (four phthalate diesters and three of their metabolites). The most abundant compounds were diethyl phthalate (DEP) and diethylhexyl phthalate (DEHP), which were quantified from 60 to 3393 ng g⁻¹ (d.w.) and from 117 to 1562 ng g⁻¹ (d.w.), respectively.

我们已经开发了一种基于加压液体萃取（PLE），然后将液相色谱与高分辨率质谱（LC-HRMS）结合的分析方法，用于同时测定广泛食用的海产品中的5种邻苯二甲酸二酯和6种邻苯二甲酸单酯。为了获得最高的提取回收率，并减少此类复杂样品的基质效应，优化了最重要的提取参数，并测试了几种净化和预浓缩策略。该方法提供的检测极限范围为1至25 ng g ^-1脂肪族和低脂族大多数化合物的干重（dw）。此外，在五种海鲜中定量了几种目标分析物（四种邻苯二甲酸二酯及其三种代谢物）。最丰富的化合物是邻苯二甲酸二乙酯（DEP）和邻苯二甲酸二乙基己酯（DEHP），其定量分别为60到3393 ng g ^-1（dw）和117到1562 ng g ^-1（dw）。