In this study, two novel mixed ACE-inhibiting peptides, namely Se-TAPep 1 and TAPep 1, were prepared and purified from selenium-enriched and common alkali-soluble proteins using a series of process, and then its physicochemical properties and oligopeptide sequence were analyzed. The results showed that Se-TAPep 1 and TAPep 1 could be successfully separated, but their ACE inhibitory activities were different (IC50 values were 13.39 mg/mL and 23.56 mg/mL, respectively), this may be related to the difference in selenium content. Further analysis of physicochemical properties showed that the protein or peptide fractions contained their characteristic absorption peaks and contained aromatic amino acids, while the apparent morphology was different, the presence of selenium could change the apparent morphology of protein or polypeptide fractions, arranging of selenium-enriched fractions more irregular. The amino acid composition analysis showed that the Pro content of the selenium-enriched fractions was higher than that of the ordinary component, whereas the oligopeptide sequence analysis showed that both LQPSLGFP derived from Se-TAPep 1 and AETGEIKGHY derived from TAPep 1 contained some characteristics of highly active ACE-inhibiting peptides, but the characteristics of LQPSLGFP were more conspicuous, which may be related to its molecular weight and source of mixed peptides. These findings indicated that the selenium-enriched tea mixed peptides had better potential uses than ordinary tea mixed peptides in the preparation of functional food.

中文翻译：

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两种不同茶碱溶性蛋白中两种新型混合ACE抑制肽的制备，理化性质和鉴定

在这项研究中，制备了两种新型的混合ACE抑制肽，即Se-TAPep 1和TAPep 1，并通过一系列过程从富硒的普通碱溶性蛋白中纯化，然后对其理化性质和寡肽序列进行了分析。分析。结果表明，Se-TAPep 1和TAPep 1可以成功分离，但它们的ACE抑制活性不同（IC50值分别为13.39 mg / mL和23.56 mg / mL），这可能与硒含量的差异有关。进一步的理化性质分析表明，蛋白质或多肽级分具有特征吸收峰和芳香族氨基酸，表观形态不同，硒的存在可以改变蛋白质或多肽级分的表观形态，富硒馏分的排列更加不规则。氨基酸组成分析表明，富硒组分的Pro含量高于普通组分，而寡肽序列分析表明，Se-TAPep 1衍生的LQPSLGFP和TAPep 1衍生的AETGEIKGHY都具有一些特征。高活性ACE抑制肽，但LQPSLGFP的特征更为明显，这可能与其分子量和混合肽来源有关。这些发现表明，富硒茶混合肽在制备功能性食品方面具有比普通茶混合肽更好的潜在用途。氨基酸组成分析表明，富硒组分的Pro含量高于普通组分，而寡肽序列分析表明，Se-TAPep 1衍生的LQPSLGFP和TAPep 1衍生的AETGEIKGHY都具有一些特征。高活性ACE抑制肽，但LQPSLGFP的特征更为明显，这可能与其分子量和混合肽来源有关。这些发现表明，富硒茶混合肽在制备功能性食品方面具有比普通茶混合肽更好的潜在用途。氨基酸组成分析表明，富硒组分的Pro含量高于普通组分，而寡肽序列分析表明，Se-TAPep 1衍生的LQPSLGFP和TAPep 1衍生的AETGEIKGHY都具有一些特征。高活性ACE抑制肽，但LQPSLGFP的特征更为明显，这可能与其分子量和混合肽来源有关。这些发现表明，富硒茶混合肽在制备功能性食品方面具有比普通茶混合肽更好的潜在用途。寡肽序列分析表明，Se-TAPep 1衍生的LQPSLGFP和TAPep 1衍生的AETGEIKGHY均具有高活性ACE抑制肽的某些特征，但LQPSLGFP的特征更为明显，这可能与其分子量和混合肽的来源。这些发现表明，富硒茶混合肽在制备功能性食品方面具有比普通茶混合肽更好的潜在用途。寡肽序列分析表明，Se-TAPep 1衍生的LQPSLGFP和TAPep 1衍生的AETGEIKGHY均具有高活性ACE抑制肽的某些特征，但LQPSLGFP的特征更为明显，这可能与其分子量和混合肽的来源。这些发现表明，富硒茶混合肽在制备功能性食品方面具有比普通茶混合肽更好的潜在用途。