Empty palm fruit bunches fiber (EPFBF) is a lignocellulosic biomass by-product of palm oil production. In the present work, EPFBF was pretreated with sequential diluted acid/alkali to enhance the material’s enzymatic digestibility. Response surface methodology (RSM) based on Central Composite Rotatable Design (CCRD) was used to optimize solids and protein loadings to maximize both glucose release and enzymatic hydrolysis (EH) yield after 48 h. Optimum solid and protein loadings determined by RSM were 243.4 g/L and 22 mg/g of cellulose, respectively, and under these conditions 74.8 ± 0.3 g/L of glucose were obtained, corresponding to an EH yield of 64.7 ± 0.2%. Polyethylene glycol 8000 (PEG 8000), bovine serum albumin (BSA), and TWEEN 20 were supplemented separately to evaluate their influence on EH of pretreated EPFBF. The highest levels of glucose release were achieved by adding 150 mg/g of cellulose of PEG 8000, increasing EH yield to 76.6 ± 0.9%. A simultaneous saccharification and fermentation (SSF) with delayed inoculation process was used for ethanol production. A final ethanol concentration of 32.6 ± 1.0 g/L was obtained with an efficiency of 74.1% and an overall productivity of 0.6 g/(L h).

空棕榈果束纤维（EPFBF）是棕榈油生产中的木质纤维素生物质副产品。在目前的工作中，EPFBF用连续的稀酸/碱进行了预处理，以增强材料的酶消化率。基于中央复合旋转设计（CCRD）的响应面方法（RSM）用于优化固体和蛋白质负载，以在48小时后最大化葡萄糖释放和酶促水解（EH）产量。通过RSM确定的最佳固体和蛋白质负载量分别为243.4 g / L和22 mg / g纤维素，在这些条件下获得74.8±0.3 g / L的葡萄糖，对应的EH收率为64.7±0.2％。分别补充了聚乙二醇8000（PEG 8000），牛血清白蛋白（BSA）和TWEEN 20，以评估它们对预处理的EPFBF的EH的影响。通过添加150 mg / g PEG 8000纤维素达到最高的葡萄糖释放水平，将EH收率提高到76.6±0.9％。延迟接种过程的同时糖化和发酵（SSF）用于生产乙醇。最终乙醇浓度为32.6±1.0 g / L，效率为74.1％，总生产率为0.6 g /（L h）。