Spatially resolved gene expression patterns are emerging as a key component of medical studies, including companion diagnostics, but technologies for quantification and multiplexing are limited. We present a method to perform spatially resolved and multiplexed microRNA (miRNA) measurements from formalin-fixed, paraffin-embedded (FFPE) tissue. Using nanoliter well arrays to pixelate the tissue section and photopatterned hydrogels to quantify miRNA, we identified differentially expressed miRNAs in tumors from a genetically engineered mouse model for non-small cell lung cancer (K-rasLSL-G12D/+; p53fl/fl). This technology could be used to quantify heterogeneities in tissue samples and lead to informed, biomarker-based diagnostics.

中文翻译：

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使用纳升孔阵列从组织中空间分辨和多路复用 MicroRNA 定量。

空间分辨基因表达模式正在成为医学研究的一个关键组成部分，包括伴随诊断，但量化和多路复用技术是有限的。我们提出了一种方法，可以从福尔马林固定、石蜡包埋 (FFPE) 组织中进行空间分辨和多路复用 microRNA (miRNA) 测量。使用纳升孔阵列来像素化组织切片和光图案化水凝胶以量化 miRNA，我们从非小细胞肺癌的基因工程小鼠模型 (K-rasLSL-G12D/+; p53fl/fl) 中鉴定出肿瘤中差异表达的 miRNA。该技术可用于量化组织样本中的异质性，并导致基于生物标志物的知情诊断。