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Characteristics of oral methicillin-resistant Staphylococcus epidermidis isolated from dental plaque.
International Journal of Oral Science ( IF 10.8 ) Pub Date : 2020-05-09 , DOI: 10.1038/s41368-020-0079-5
Boyu Tang 1 , Tao Gong 1 , Yujia Cui 1 , Lingyun Wang 2 , Chao He 3 , Miao Lu 1 , Jiamin Chen 1 , Meiling Jing 1 , Anqi Zhang 1 , Yuqing Li 1
Affiliation  

The oral microbial community is widely regarded as a latent reservoir of antibiotic resistance genes. This study assessed the molecular epidemiology, susceptibility profile, and resistance mechanisms of 35 methicillin-resistant Staphylococcus epidermidis (MRSE) strains isolated from the dental plaque of a healthy human population. Broth microdilution minimum inhibitory concentrations (MICs) revealed that all the isolates were nonsusceptible to oxacillin and penicillin G. Most of them were also resistant to trimethoprim (65.7%) and erythromycin (54.3%). The resistance to multiple antibiotics was found to be largely due to the acquisition of plasmid-borne genes. The mecA and dfrA genes were found in all the isolates, mostly dfrG (80%), aacA-aphD (20%), aadD (28.6%), aphA3 (22.9%), msrA (5.7%), and the ermC gene (14.3%). Classical mutational mechanisms found in these isolates were mainly efflux pumps such as qacA (31.4%), qacC (25.7%), tetK (17.1%), and norA (8.6%). Multilocus sequence type analysis revealed that sequence type 59 (ST59) strains comprised 71.43% of the typed isolates, and the eBURST algorithm clustered STs into the clonal complex 2-II(CC2-II). The staphyloccoccal cassette chromosome mec (SCCmec) type results showed that 25 (71.43%) were assigned to type IV. Moreover, 88.66% of the isolates were found to harbor six or more biofilm-associated genes. The aap, atlE, embp, sdrF, and IS256 genes were detected in all 35 isolates. This research demonstrates that biofilm-positive multiple-antibiotic-resistant ST59-SCCmec IV S. epidermidis strains exist in the dental plaque of healthy people and may be a potential risk for the transmission of antibiotic resistance.

中文翻译:

从牙菌斑中分离出的耐甲氧西林的表皮葡萄球菌的特征。

口腔微生物群落被广泛认为是抗生素抗性基因的潜在库。这项研究评估了从健康人群的牙菌斑中分离出的35种耐甲氧西林表皮葡萄球菌(MRSE)菌株的分子流行病学,药敏谱和耐药机制。肉汤微量稀释最低抑菌浓度(MICs)显示,所有分离株对奥沙西林和青霉素G不敏感。它们中的大多数还对甲氧苄啶(65.7%)和红霉素(54.3%)耐药。发现对多种抗生素的抗性在很大程度上归因于质粒载体基因的获得。在所有分离物中都发现了mecA和dfrA基因,主要是dfrG(80%),aacA-aphD(20%),aadD(28.6%),aphA3(22.9%),msrA(5.7%)和ermC基因( 14.3%)。在这些分离物中发现的经典突变机制主要是外排泵,例如qacA(31.4%),qacC(25.7%),tetK(17.1%)和norA(8.6%)。多基因座序列类型分析表明,序列类型59(ST59)菌株占类型分离株的71.43%,eBURST算法将ST聚类为克隆复合物2-II(CC2-II)。结果显示,葡萄球菌盒式染色体mec(SCCmec)类型为25,占71.43%。此外,发现88.86%的分离物具有六个或更多与生物膜相关的基因。在所有35个分离株中均检测到aap,atlE,emp,sdrF和IS256基因。这项研究表明生物膜阳性多重耐药ST59-SCCmec IVS。
更新日期:2020-05-09
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