Quantitative proteomic studies are contributing greatly to the understanding of the spermatozoon through the provision of detailed information on the proteins spermatozoa acquire and shed in the acquisition of fertility. Extracellular vesicles (EVs) are thought to aid in the delivery of proteins to spermatozoa in the male reproductive tract. The aim of this study is to isolate, identify and quantify EV proteins isolated from ram seminal plasma. Ram sperm plasma membrane proteins are also isolated using nitrogen cavitation and identified to better understand the interplay of proteins between the sperm membrane and extracellular environment. The categorization of proteins enriched in the EV population according to their function revealed three main groupings: vesicle biogenesis, metabolism, and membrane adhesion and remodeling. The latter group contains many reproduction‐specific proteins that show demonstrable links to sperm fertility. Many of these membrane‐bound proteins show testicular expression and are shed from the sperm surface during epididymal maturation (e.g., testis expressed 101; TEX101 and lymphocyte Antigen 6 Family Member K; LY6K). Their association with seminal EVs suggests that EVs may not only deliver protein cargo to spermatozoa but also assist in the removal of proteins from the sperm membrane.

中文翻译：

---

精浆，精子膜蛋白和精子胞外囊泡的定量蛋白质组学分析表明，囊泡机制有助于去除和添加蛋白质到Ram精子膜。

蛋白质组学的定量研究通过提供有关精子获取和在获得生育能力中脱落的蛋白质的详细信息，极大地促进了对精子的理解。人们认为细胞外囊泡（EVs）有助于将蛋白质输送到男性生殖道中的精子。这项研究的目的是分离，鉴定和定量从精浆中分离出的EV蛋白。Ram精子质膜蛋白也可以通过氮气空化来分离，并经过鉴定可以更好地了解精子膜与细胞外环境之间的相互作用。根据其功能，富集在EV人群中的蛋白质的分类揭示了三个主要类别：囊泡的生物发生，代谢以及膜粘附和重塑。后一组含有许多生殖特异性蛋白，这些蛋白与精子的受精率有明显关系。这些许多膜结合蛋白在附睾成熟期间显示睾丸表达并从精子表面脱落（例如，睾丸表达101； TEX101和淋巴细胞抗原6家族成员K； LY6K）。他们与精液电动车的联系表明，电动车不仅可以将蛋白质运送到精子，还可以帮助从精子膜中去除蛋白质。