The stability of the gossypol amine adducts used for chromatographic determination of gossypol was studied. After extraction and complexation with R ‐(−)‐2‐amino‐1‐propanol, the samples were diluted into an acetonitrile/phosphate buffer solution as described in AOCS Recommended Practice Ba 8a‐99. The solutions were then stored under different conditions before being analyzed by reverse‐phase chromatography. Samples stored in the dark at −80 °C or at −20 °C showed little change in peak size over 30 days. Samples stored in the dark at −4 °C or at room temperature showed a measurable reduction in gossypol peak size over the study period. Samples stored in the light at room temperature showed the greatest reduction with only 25% of the initial gossypol detectable after 30 days. The rate of degradation followed first‐order kinetics. The rate of decrease in gossypol peak size did not differ for the different gossypol matrices studied, i.e., cottonseed kernels, cottonseed meal, or pure gossypol‐acetic acid; nor did it differ for the individual gossypol enantiomers. The results indicate that these gossypol Schiff's base adducts can be transported on dry ice before chromatography with minimal concern for their stability.

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用于总和异构棉酚色谱测量的棉酚胺加合物的稳定性

研究了用于色谱分析棉酚的棉酚胺加合物的稳定性。提取并与R络合后-（-）-2-氨基-1-丙醇，将样品稀释到乙腈/磷酸盐缓冲溶液中，如AOCS建议操作Ba 8a-99中所述。然后将溶液保存在不同的条件下，然后进行反相色谱分析。在−80°C或−20°C的黑暗环境中存储的样品在30天内的峰大小几乎没有变化。在研究期内，在−4°C或室温下避光保存的样品显示出棉酚峰大小的可测量减少。室温下储存在光线下的样品显示最大的减少，在30天后仅检测到初始棉酚的25％。降解速率遵循一级动力学。对于所研究的不同棉酚基质，棉籽粒，棉籽粕，棉籽粕，棉籽粕，棉籽粕，或纯棉酚乙酸；棉酚对映体的个体也没有区别。结果表明，这些棉酚席夫氏碱加合物可以在层析之前在干冰上运输，而对其稳定性的关注最小。