Abstract Supported lipid bilayers (SLBs) spanning hydrophilic surfaces are industrially attractive biomimetic coatings that mimic critical aspects of lipid membrane interfaces and are increasingly used in applications spanning medicine, biotechnology, and environmental science. The use of adsorbing bicelle lipid nanostructures composed of long- and short-chain phospholipid mixtures is an effective self-assembly driven process for streamlined SLB fabrication. However, existing studies use synthetic short-chain phospholipids as a necessary bicelle component and such materials are not practical for industrial applications. Herein, we investigated optimal conditions to fabricate SLBs from bicelles containing an industrially useful monoglyceride called monocaprin (MC) in place of short-chain phospholipids. The ratio of long-chain phospholipid to MC along with total lipid concentration were systematically tested. Quartz crystal microbalance-dissipation (QCM-D) and time-lapse fluorescence microscopy experiments were performed to characterize bicelle adsorption onto silicon dioxide surfaces, and fluorescence recovery after photobleaching (FRAP) measurements were conducted to evaluate lateral lipid diffusion within the fabricated lipid adlayers. Depending on bicelle parameters, high-quality SLB formation with uniform phase properties was achieved and optimal ranges are described to ensure target performance outcomes without phase separation. Together, our findings demonstrate that MC-containing bicelles are useful tools to form high-quality SLBs suitable for surface coating and biosensing applications.

中文翻译：

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从磷脂-单甘油酯双胞胎混合物中最佳形成均匀相支持的脂质双层

摘要 跨越亲水表面的支撑脂质双层 (SLB) 是具有工业吸引力的仿生涂层，可模拟脂质膜界面的关键方面，并越来越多地用于跨越医学、生物技术和环境科学的应用。使用由长链和短链磷脂混合物组成的吸附双细胞脂质纳米结构是一种有效的自组装驱动过程，可用于简化 SLB 制造。然而，现有的研究使用合成的短链磷脂作为必要的双胞胎成分，这种材料在工业应用中并不实用。在此，我们研究了从含有工业上有用的甘油单酯（称为单癸酸（MC））代替短链磷脂的双细胞制造 SLB 的最佳条件。系统地测试了长链磷脂与 MC 的比例以及总脂质浓度。进行了石英晶体微量天平消散 (QCM-D) 和延时荧光显微镜实验，以表征二氧化硅表面上的 Bicelle 吸附，并进行光漂白 (FRAP) 测量后的荧光恢复，以评估制造的脂质层内的横向脂质扩散。根据 bicelle 参数，实现了具有均匀相特性的高质量 SLB 形成，并描述了最佳范围以确保目标性能结果而不会发生相分离。总之，我们的研究结果表明，含 MC 的 bicelles 是形成适用于表面涂层和生物传感应用的高质量 SLB 的有用工具。