Green synthesis of silver nanoparticles has gained great interest among scientists. In view of this data, we conducted this study to identify the ameliorative effect of green synthesis of silver nanoparticles using Nigella sativa extract in diabetic neuropathy induced experimentally. In this study, 50 adult male albino rats were used and they were randomly divided into five groups; the first group was the healthy control group, the second group were the diabetic neuropathy diabetic neuropathy induced, Groups (3-6) diabetic neuropathy induced group and treated with silver nanoparticles, Nigella sativa extract and green synthesized silver nanoparticles using Nigella sativa extract respectively. Biochemical parameters including diabetic, inflammatory and antioxidant biomarkers were evaluated. Brain histopathology was also performed. Results revealed substantial rise in glucose, AGE, aldose reductase with insulin reduction in diabetic neuropathy induced group as compared to healthy control. Also, inflammatory markers increased significantly in diabetic neuropathy induced group. A remarkable change in oxidative status was observed in the same group. Furthermore, significant decline in nitrotyrosin level was observed. Regarding gene expression, we found significant down regulation in brain TKr A accompanied by upregulation of nerve growth factor in diabetic neuropathy group comparing with healthy control. Several treatments for diabetic neuropathy remarkably ameliorate all the investigated biomarkers. Histological findings are greatly relied on for the results achieved in this study. Therefore, it can be established that green synthesis of silver nanoparticles in combination with Nigella sativa extract could be a newly neuroprotective agents against inflammation and oxidative stress characterizing diabetic neuropathy through their antidiabetic, anti-inflammatory and anti-oxidants effects.

银纳米颗粒的绿色合成引起了科学家的极大兴趣。鉴于这些数据，我们进行本研究以确定使用银纳米颗粒的绿色合成的改善效果果黑种草提取物在实验性诱导的糖尿病性神经病变。在这项研究中，使用50只成年雄性白化病雄性大鼠，将其随机分为5组；每组5只。第一组是健康对照组，第二组是糖尿病性神经病引起的糖尿病性神经病，第（3-6）组是糖尿病性神经病引起的组，并用银纳米颗粒，紫菜提取物和使用紫菜的绿色合成银纳米颗粒治疗分别提取。评价了包括糖尿病，炎症和抗氧化剂生物标志物在内的生化参数。还进行了脑组织病理学检查。结果显示，与健康对照组相比，糖尿病神经病变诱导组的葡萄糖，AGE，醛糖还原酶显着升高，且胰岛素减少。同样，在糖尿病性神经病诱导组中，炎症标志物显着增加。在同一组中观察到氧化状态的显着变化。此外，观察到硝基酪氨酸水平显着下降。关于基因表达，我们发现与健康对照组相比，糖尿病神经病变组的脑TKr A明显下调，并伴有神经生长因子的上调。几种治疗糖尿病性神经病的方法可明显改善所有研究的生物标志物。组织学的发现很大程度上取决于这项研究的结果。因此，可以确定银纳米颗粒与苜蓿提取物可能具有抗糖尿病，抗炎和抗氧化剂的作用，是一种针对炎症和氧化应激的新型神经保护剂，可表征糖尿病性神经病。