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Effects of 17α‐methyltestosterone feeding and immersion on masculinization of rosy barbs Pethia conchonius and dwarf gouramis Trichogaster lalius
Journal of the World Aquaculture Society ( IF 2.3 ) Pub Date : 2020-05-07 , DOI: 10.1111/jwas.12703
Shane W. Ramee 1 , Taylor N. Lipscomb 1 , Matthew A. DiMaggio 1
Affiliation  

Hormone treatments have commonly been used to skew sex ratios toward desired phenotypes. In sexually dichromic ornamental species, males are often more ornately colored and therefore more valuable. The synthetic androgen 17α‐methyltestosterone (MT) is one of the most common hormones used in this practice. The rosy barb, Pethia conchonius, and dwarf gourami, Trichogaster lalius, are two sexually dichromic species that would benefit from masculinization. The larvae of T. lalius and Pethia conchonius were exposed to MT over the first 30–60 days posthatch either through a series of 4‐hr immersions (0, 100, 200, 400, or 800 μg MT/L) or via top‐coated feed (0, 15, 30, 60, 120, 150, or 250 mg MT/kg feed). Survival was determined at the end of each treatment regime, and sex ratios were determined via gross dissections. In rosy barbs, none of the 30‐day treatments resulted in significant masculinization. MT feeding for 60 days resulted in more than 85% males in P. conchonius, but this was not significantly different from the control (70%). In T. lalius, paradoxical feminization was observed in immersion concentrations and 50 days of MT feed treatments.

中文翻译:

17α-甲基睾丸激素的摄食和浸没对玫瑰色倒钩Pethia conhonius和矮矮牵牛Trichogaster lalius男性化的影响

激素治疗通常用于使性别比例偏向所需的表型。在有色双色的观赏物种中,雄性的颜色通常更华丽,因此更有价值。合成雄激素17α-甲基睾丸激素(MT)是该实践中最常用的激素之一。玫瑰色的倒钩Pethia conchonius和矮生的古拉米Trichogaster lalius是两个有性双色种,可以从男性化中受益。T. laliusPethia conhonius的幼虫在孵化后的头30–60天通过一系列4个小时的浸泡(0、100、200、400或800μgMT / L)或通过表面包衣的饲料(0、15、30, 60、120、150或250 mg MT / kg饲料)。在每种治疗方案结束时确定存活率,并通过大体解剖确定性别比。在玫瑰色的倒钩中,30天的治疗均未导致明显的男性化。60天的MT喂养导致孔雀假单胞菌中超过85%的雄性,但这与对照(70%)没有显着差异。在T. lalius中,在浸泡浓度和MT饲料处理的50天中观察到了矛盾的女性化现象。
更新日期:2020-05-07
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