Mapping of neuropeptide Y expression in Octopus brains,Journal of Morphology

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Mapping of neuropeptide Y expression in Octopus brains
Journal of Morphology ( IF 1.5 ) Pub Date : 2020-05-08 , DOI: 10.1002/jmor.21141
Gabrielle C Winters ₁ , Gianluca Polese ₂ , Anna Di Cosmo ₂ , Leonid L Moroz _{1,

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Affiliation

Neuropeptide Y (NPY) is an evolutionarily conserved neurosecretory molecule implicated in a diverse complement of functions across taxa and in regulating feeding behavior and reproductive maturation in Octopus. However, little is known about the precise molecular circuitry of NPY‐mediated behaviors and physiological processes, which likely involve a complex interaction of multiple signal molecules in specific brain regions. Here, we examined the expression of NPY throughout the Octopus central nervous system. The sequence analysis of Octopus NPY precursor confirmed the presence of both, signal peptide and putative active peptides, which are highly conserved across bilaterians. In situ hybridization revealed distinct expression of NPY in specialized compartments, including potential “integration centers,” where visual, tactile, and other behavioral circuitries converge. These centers integrating separate circuits may maintain and modulate learning and memory or other behaviors not yet attributed to NPY‐dependent modulation in Octopus. Extrasomatic localization of NPY mRNA in the neurites of specific neuron populations in the brain suggests a potential demand for immediate translation at synapses and a crucial temporal role for NPY in these cell populations. We also documented the presence of NPY mRNA in a small cell population in the olfactory lobe, which is a component of the Octopus feeding and reproductive control centers. However, the molecular mapping of NPY expression only partially overlapped with that produced by immunohistochemistry in previous studies. Our study provides a precise molecular map of NPY mRNA expression that can be used to design and test future hypotheses about molecular signaling in various Octopus behaviors.

中文翻译：

章鱼脑中神经肽 Y 表达的定位

神经肽 Y (NPY) 是一种进化上保守的神经分泌分子，涉及整个分类群的多种功能补充以及调节章鱼的摄食行为和生殖成熟。然而，人们对 NPY 介导的行为和生理过程的精确分子电路知之甚少，这可能涉及特定大脑区域中多个信号分子的复杂相互作用。在这里，我们检查了整个章鱼中枢神经系统中 NPY 的表达。Octopus NPY 前体的序列分析证实了信号肽和推定的活性肽的存在，它们在两侧高度保守。原位杂交揭示了 NPY 在特殊隔室中的不同表达，包括潜在的“整合中心”，其中视觉、触觉、和其他行为电路收敛。这些集成独立电路的中心可以维持和调节学习和记忆或其他尚未归因于章鱼中 NPY 依赖性调制的行为。NPY mRNA 在大脑中特定神经元群的神经突中的体外定位表明在突触处立即翻译的潜在需求以及 NPY 在这些细胞群中的关键时间作用。我们还记录了嗅叶小细胞群中 NPY mRNA 的存在，嗅叶是章鱼摄食和生殖控制中心的一个组成部分。然而，NPY 表达的分子图谱与之前研究中免疫组织化学产生的分子图谱仅部分重叠。

更新日期：2020-05-08

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