The ability to identify the sex of embryo and control of sex ratio has a great commercial importance to livestock industry. Prediction of embryonic sex could be useful in the management decisions of sex selection in breeding programs. Several methods have been attempted to determine the sex but the polymerase chain reaction (PCR)‐based sexing method is generally favoured, as it is cost effective, simple and reliable. The aim of the present study was to identify sex of sheep embryos produced in vitro through amplification of glyceraldehyde 3‐phosphate dehydrogenase (GAPDH), sex‐determining region Y (SRY) and amelogenin genes present in genomic DNA (gDNA) of embryos through PCR. To avoid false interpretation of the result by no amplification of SRY in female embryos, a duplex PCR was approached to amplify combinedly SRY and GAPDH genes. Sex‐specific blood was used in PCR as positive control. In vitro sheep embryos were produced as per standardized protocol of laboratory. Sexing of sex‐specific blood and in vitro produced embryos were approached though PCR to amplify the respective genes using gDNA present in the sample without its traditional isolation. The accuracy of sex prediction for embryos was 100% by this procedure.

中文翻译：

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通过基于聚合酶链反应的GAPDH，SRY和AMEL基因扩增，对植入前的绵羊胚胎进行性别鉴定。

识别胚胎性别和控制性别比例的能力对畜牧业具有重要的商业意义。胚胎性别的预测可能对育种计划中性别选择的管理决策有用。已经尝试了几种方法来确定性别，但是基于聚合酶链反应（PCR）的性别鉴定方法通常是受青睐的，因为它具有成本效益，简单且可靠的特点。本研究的目的是通过PCR扩增3-醛酸甘油三磷酸脱氢酶（GAPDH），性别决定区Y（SRY）和牙釉蛋白基因（存在于胚胎的基因组DNA中）来鉴定体外产生的绵羊胚胎的性别。 。为了避免在雌性胚胎中不扩增SRY而对结果产生错误的解释，采用双链PCR扩增SRY和GAPDH基因。PCR中使用了具有性别特异性的血液作为阳性对照。按照实验室的标准化方案生产了体外绵羊胚胎。通过PCR进行性别特异性血液和体外产生的胚胎的性别鉴定，以使用样品中存在的gDNA扩增相应基因，而无需传统分离。通过此程序，对胚胎进行性别预测的准确性为100％。