The tapetum plays a crucial role in pollen development by nursing and releasing the microspore. The tapetum undergoes programmed cell death (PCD), and appropriate timing of PCD is essential for microsporogenesis. Undeveloped Tapetum1 (UDT1) is known to function in anther development, but the regulation mechanism of tapetum differentiation and degeneration by UDT1 is largely unknown. Through comparative transcriptome analysis, we selected 100 genes as udt1 downregulated genes. The biological process related to the negative regulation of translation is the most enriched in udt1 downregulated genes. It is attributed by ribosome inactivating proteins (RIPs), but the role of RIPs is not well defined, and they are assumed to participate in tapetal PCD. Lipid transport is another overrepresented Gene Ontology (GO) term in udt1 downregulated genes, indicating the functional loss of the tapetum as the nursing cell. Using comparative analysis and real-time PCR, we infer that UDT1 can trigger tapetal PCD by controlling the expression of RIPs and three aspartyl proteases (AP) grouped with OsAP37, which is well known as being involved in tapetum degeneration.

中文翻译：

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比较转录组分析揭示了UDT1对花药发育的基因调控机制。

绒毡层通过护理和释放小孢子在花粉发育中起关键作用。绒毡层经历了程序性细胞死亡（PCD），并且PCD的适当时机对于微孢子发生至关重要。尚未开发的Tapetum1（UDT1）在花药发育中起作用，但是UDT1对绒毡层分化和变性的调控机制尚不清楚。通过比较转录组分析，我们选择了100个基因作为udt1下调基因。与翻译负调控有关的生物学过程在udt1中最丰富下调的基因。它归因于核糖体失活蛋白（RIPs），但RIPs的作用尚未明确定义，并假定它们参与了绒毡状PCD。脂质转运是udt1下调基因中另一个过分代表的基因本体论（GO）术语，表明绒毡层作为哺乳细胞的功能丧失。使用比较分析和实时PCR，我们推断UDT1可以通过控制与OsAP37结合的RIP和三种天冬氨酰蛋白酶（AP）的表达来触发绒毡状PCD，这被认为与绒毡层变性有关。