Pseudorabies virus (PRV) belongs to the Alphaherpesvirinae subfamily of Herpesviridae. PRV-induced pseudorabies is a highly contagious disease that has caused huge economic losses to the global swine industry. The PRV gE/gI gene deletion vaccine strain (Fa ΔgE/gI strain) constructed from the PRV Fa wild-type strain was shown to have a protective effect against infection. However, the interaction between PRV gE/gI genes and host miRNA needs further exploration, and little is known about the regulatory mechanisms of non-coding RNAs during PRV infection. miRNAs play a key regulatory role in viral infection and immune responses, so we analyzed the differential expression of miRNAs induced by the PRV Fa ΔgE/gI strain and Fa wild-type strain in the PK15 cell line. High-throughput sequencing reads were aligned to known Sus scrofa pre-miRNAs in the miRBase database. Target genes of differentially expressed miRNAs were predicted using the miRGen 3.0 database, then filtered miRNA target genes were subjected to Gene Ontology (GO) analysis and Search Tool for the Retrieval of Interacting Genes/ Proteins (STRING) analysis. Stem-loop quantitative real-time PCR was performed to confirm the accuracy of high-throughput sequencing data. In total, 387, 472, and 490 annotated and novel mature miRNAs were identified from PRV Fa ΔgE/gI strain-infected, Fa wild-type strain-infected, and non-infected PK-15 cells, respectively. Five PRV-encoded miRNAs were also identified. GO analysis showed that target genes of differentially expressed miRNAs in PRV Fa ΔgE/gI strain-infected and Fa wild-type strain-infected PK-15 cells were mainly involved in biological regulation and metabolic processes. STRING analysis showed that immune-related target genes of differentially expressed miRNAs in the Toll-like receptor signaling pathway, B cell receptor signaling pathway, T cell receptor signaling pathway, nuclear factor-κB signaling pathway, and transforming growth factor-β signaling pathway were interrelated. This is the first report of the small RNA transcriptome in PRV mutant wild-type strain-infected and Fa ΔgE/gI strain-infected porcine cell lines. Our findings will contribute to the prevention and treatment of PRV mutant strains.

中文翻译：

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gE / gI缺失对PRV感染的PK-15细胞miRNA表达的影响。

伪狂犬病病毒（PRV）属于疱疹病毒科的Alphaherpesvirinae亚科。PRV引起的假狂犬病是一种高度传染性疾病，已给全球养猪业造成巨大的经济损失。由PRV Fa野生型菌株构建的PRV gE / gI基因缺失疫苗株（FaΔgE/ gI株）显示出对感染的保护作用。然而，PRV gE / gI基因与宿主miRNA之间的相互作用尚需进一步探索，关于PRV感染过程中非编码RNA的调控机制知之甚少。miRNA在病毒感染和免疫反应中起关键的调节作用，因此我们分析了PRV FaΔgE/ gI株和Fa野生型株在PK15细胞系中诱导的miRNA差异表达。将高通量测序读数与miRBase数据库中已知的Sus scrofa pre-miRNA进行比对。使用miRGen 3.0数据库预测差异表达的miRNA的靶基因，然后对筛选的miRNA靶基因进行基因本体（GO）分析和检索工具以检索相互作用的基因/蛋白质（STRING）分析。进行茎环定量实时PCR以确认高通量测序数据的准确性。总共分别从感染PRV FaΔgE/ gI株，感染Fa的野生型株和未感染PK-15细胞中鉴定了387、472和490条带注释的新的成熟miRNA。还鉴定了五个PRV编码的miRNA。GO分析表明，PRV FaΔgE/ gI株感染和Fa野生型株感染的PK-15细胞中差异表达的miRNA的靶基因主要参与生物学调控和代谢过程。STRING分析显示，Toll样受体信号传导途径，B细胞受体信号传导途径，T细胞受体信号传导途径，核因子-κB信号传导途径和转化生长因子-β信号传导途径中差异表达的miRNA的免疫相关靶基因分别为相关。这是PRV突变野生型菌株感染和FaΔgE/ gI菌株感染的猪细胞系中小RNA转录组的首次报道。我们的发现将有助于预防和治疗PRV突变株。STRING分析显示，Toll样受体信号传导途径，B细胞受体信号传导途径，T细胞受体信号传导途径，核因子-κB信号传导途径和转化生长因子-β信号传导途径中差异表达的miRNA的免疫相关靶基因分别为相关。这是PRV突变野生型菌株感染和FaΔgE/ gI菌株感染的猪细胞系中小RNA转录组的首次报道。我们的发现将有助于预防和治疗PRV突变株。STRING分析显示，Toll样受体信号传导途径，B细胞受体信号传导途径，T细胞受体信号传导途径，核因子-κB信号传导途径和转化生长因子-β信号传导途径中差异表达的miRNA的免疫相关靶基因分别为相关。这是PRV突变野生型菌株感染的和FaΔgE/ gI菌株感染的猪细胞系中小RNA转录组的首次报道。我们的发现将有助于预防和治疗PRV突变株。这是PRV突变野生型菌株感染和FaΔgE/ gI菌株感染的猪细胞系中小RNA转录组的首次报道。我们的发现将有助于预防和治疗PRV突变株。这是PRV突变野生型菌株感染和FaΔgE/ gI菌株感染的猪细胞系中小RNA转录组的首次报道。我们的发现将有助于预防和治疗PRV突变株。