To verify the influence of ozone (O3) therapy on an experimental model of rheumatoid arthritis (RA), 30 male Wistar rats were randomly allocated to 2 groups, control (C) and treatment (T), and subdivided into control (C12, C48, C72) and treatment (T12, T48, T72) groups. RA was induced by administration of collagenase plus complete Freud's adjuvant in the knee joint region. The animals were treated with ozone therapy (1 ml O3 injection in the knee i.a.) according to group assignment: T12, 2 h; T48, 2 and 24 h; and T72, 2, 24, and 48 h post-RA induction. The different animal groups were euthanized 12, 24, or 72 h post-RA induction, respectively. Synovial exudate levels of IL-10, IL-12p70, TNF-α, INF-γ, and MCP-1 were assessed by flow cytometry, and histopathological analysis of the knee cartilage was conducted. Ozone therapy effectively decreases inflammation, reducing IL-12 and TNF-α, and increasing IL10. O3 did not statistically affect INF-γ or MCP-1 levels. More expressive results were obtained with group T72, i.e., treated 2, 24, and 48 h post-RA induction, which indicates that longer-term ozone treatment is more effective than a single acute application. Ozone therapy effectively reduced inflammation with effects, at least in part, mediated through reduction of pro-inflammatory cytokines and activation of IL-10 anti-inflammatory cytokine.

中文翻译：

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臭氧疗法在类风湿关节炎实验模型中的抗炎作用。

为了验证臭氧（O3）治疗对类风湿性关节炎（RA）实验模型的影响，将30只雄性Wistar大鼠随机分为两组，即对照组（C）和治疗（T），并细分为对照组（C12，C48 ，C72）和治疗（T12，T48，T72）组。通过在膝关节区域施用胶原酶和完全弗洛伊德佐剂诱导RA。根据组分配：T12，2 h，用臭氧疗法（膝盖内注射1 ml O3）对动物进行治疗。T48、2和24小时；RA诱导后的T72、2、24和48小时。RA诱导后分别在12、24或72 h对不同的动物组实施安乐死。通过流式细胞术评估滑膜分泌液中IL-10，IL-12p70，TNF-α，INF-γ和MCP-1的水平，并对膝关节软骨进行组织病理学分析。臭氧疗法可有效减少炎症，减少IL-12和TNF-α并增加IL10。O3没有统计学上影响INF-γ或MCP-1的水平。在T72组中，即在RA诱导后第2、24和48 h处理后，获得了更具表达力的结果，这表明长期臭氧处理比单次急性应用更为有效。臭氧疗法通过减少促炎细胞因子和激活IL-10抗炎细胞因子，至少部分地有效减轻了炎症。这表明长期臭氧处理比单次急性应用更为有效。臭氧疗法通过减少促炎细胞因子和激活IL-10抗炎细胞因子，至少部分地有效减轻了炎症。这表明长期臭氧处理比单次急性应用更为有效。臭氧疗法通过减少促炎细胞因子和激活IL-10抗炎细胞因子，至少部分地有效减轻了炎症。