Potato virus Y (PVY) is a major pathogen affecting potato production worldwide. Three independent genes (Ry_adg,Ry_sto,and Ry_chc) conferring genetic resistance to all known strains of the virus are currently utilized by breeding programs to develop potato varieties containing extreme resistance to infection by PVY. These resistance genes are typically detected using primers specific for the generation of markers closely-linked to the resistance genes. In recent years there have been attempts to develop a multiplex PCR assay for all resistance genes, but all current published methods either omit at least one resistance gene or are unnecessarily complex and therefore difficult to incorporate successfully for marker-assisted selection. We have developed an assay which tests for the presence of all three sources of resistance in a single, easy to use PCR protocol. The multiplex PCR assay was applied to potato varieties, second-field year breeding germplasm, and a selection of test crosses having progeny segregating for multiple resistance genes to validate the methodology for use in marker-assisted selection in potato breeding programs.

中文翻译：

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检测马铃薯马铃薯PVY抗性基因的多重标记测定方法的开发与应用

马铃薯病毒Y（PVY）是影响全世界马铃薯生产的主要病原体。三个独立的基因（Ry _adg， Ry _sto和Ry _chc育种计划目前正在利用对所有已知病毒株具有遗传抗性的马铃薯来开发对PVY感染具有极强抗性的马铃薯品种。这些抗性基因通常使用特异于与抗性基因紧密连接的标记的产生的引物来检测。近年来，已经尝试开发针对所有抗性基因的多重PCR测定法，但是所有当前公开的方法要么省略了至少一个抗性基因，要么不必要地复杂，因此难以成功地用于标记辅助选择。我们开发了一种检测方法，可在一个简单易用的PCR方案中检测所有三种耐药源的存在。多重PCR检测法适用于马铃薯品种，第二年育种种质，