Bio-fabrication of metal nanocrystals can be achieved using eco-friendly and cost-effective routes with plants as reducing and capping mediators. A rapid and simple method for producing silver nanoparticles (AgNPs) using the plant Alstonia venenata (R. Br.) (Family: Apocynaceae) was investigated. The mosquito larvicidal potential and the effect of the A. venenata aqueous leaf extract and AgNPs on non-target fish and insects were evaluated. The AgNPs were studied using UV–Vis spectroscopy, FTIR spectroscopy, SEM, TEM, AFM, and XRD analysis. The larvicidal effectiveness on early third instar larvae was higher for the AgNPs than the plant extract; this was observed by testing on Anopheles stephensi (LC₅₀ = 12.28 µg/mL), Aedes aegypti (LC₅₀ = 13.49 µg/mL), and Culex quinquefasciatus (LC₅₀ = 14.50 µg/mL). Furthermore, the plant extract and AgNPs were found to be safe for the environment-friendly Gambusia affinis fish, and Anisops bouvieri and Diplonychus indicus aquatic insects. This study confirmed that A. venenata is a potential bio-resource for the fabrication of nanocrystals as an effective mosquito control tool with negligible harmful on aquatic fish and insects in the environment.

金属纳米晶体的生物制造可以使用植物作为还原和封端介体的环保且经济高效的途径来实现。研究了使用植物Alstonia venenata（R. Br。）（家族：夹竹桃科）生产银纳米颗粒（AgNPs）的快速简便方法。评估了蚊子的杀幼虫潜力以及A. venenata水叶提取物和AgNPs对非目标鱼类和昆虫的影响。使用紫外-可见光谱，FTIR光谱，SEM，TEM，AFM和XRD分析研究了AgNPs。AgNPs对早期三龄幼虫的杀幼虫效果要高于植物提取物。通过在斯蒂芬按蚊（LC ₅₀  = 12.28 µg / mL）中进行测试可以观察到这一点，埃及伊蚊（LC ₅₀  = 13.49 µg / mL）和库蚊（Culex quinquefasciatus）（LC ₅₀  = 14.50 µg / mL）。此外，植物提取物和的AgNPs被认为是对环境友好安全的食蚊鱼，Anisops bouvieri和Diplonychus蜓水生昆虫。这项研究证实，A。venenata是一种潜在的生物资源，可用于制造纳米晶体，将其作为一种有效的灭蚊工具，对环境中的水生鱼类和昆虫的危害可忽略不计。