This study was conducted to evaluate expression of 11 genes, endochitinase (chit33), endochitinase (chit37), endochitinase (chit42), N-acetyl-B-D-glucosaminidase (nag1), α-1,2-mannosidase (gh92), α-1,3-glucanase (a13gluc), β-1,6-glucanase (b16gluc), β-1,3-glucanase (b13gluc), aminopeptidase S9 (s9), trypsin-like (pra1) and serine protease (sprt) which may be involved in biological control of Sclerotinia sclerotiorum by Trichoderma viridescens. Total RNA was extracted from the mycelia where colonies of the pathogen and T. viridescens reached together on potato dextrose agar. As a control, a confrontation assay was conducted where T. viridescens was interacted with itself. The results of reverse transcriptase-PCR detected no band for s9 and sprt. However, expression of a13gluc, b16gluc, chit33 and chit37 was upregulated. No change in expressions was detected in chit42, b13gluc and pra1 compared with the control. The gh92 was upregulated only in one isolate (Tvig2). The expression of nag1 in both isolates of T. viridescens was clearly downregulated compared with the control. This study showed that upregulation of a13gluc, b16gluc, chit33 and chit37 in T. viridescens may play a role in biocontrol of S. sclerotiorum.

这项研究旨在评估11种基因的表达，即内切几丁质酶（chit33），内切几丁质酶（chit37），内切几丁质酶（chit42），N-乙酰基BD-氨基葡萄糖苷酶（nag1），α-1,2-甘露糖苷酶（gh92），α- 1,3-葡聚糖酶（a13gluc），β-​​1,6-葡聚糖酶（b16gluc），β-​​1,3-葡聚糖酶（b13gluc），氨肽酶S9（s9），类胰蛋白酶（pra1）和丝氨酸蛋白酶（sprt）可能参与了木霉菌的生物控制菌核盘菌。从菌丝体中提取总RNA，在该菌丝体上，病原菌和杀伤性锥虫在马铃薯葡萄糖琼脂上一起到达菌落。作为对照，进行了对抗试验，在该试验中，病毒小球菌与其自身相互作用。逆转录-PCR的结果未检测到s9和sprt的条带。但是，a13gluc，b16gluc，chit33和chit37的表达上调。与对照相比，在chit42，b13gluc和pra1中未检测到表达变化。该gh92仅在一种分离株（Tvig2）中被上调。与对照相比，在两个T. viridescens分离株中nag1的表达明显下调。这项研究显示的是上调a13gluc，b16gluc，chit33和chit37在T. viridescens可以在生物防治中发挥作用菌核病。