To prevent infections local delivery of antibiotics is a useful tool. Especially in bone fractures, vancomycin impregnated bone cements are often used allowing high concentrations of antibiotics at the infection side without high serum concentrations. However, besides potential pathogens, cells involved in tissue regeneration may also be affected by the drug. We investigated the effect of vancomycin on the viability and functionality on osteoblasts, endothelial cells, fibroblasts and skeletal muscle cells. Our results show that the viability of all cells analyzed was reduced by vancomycin and that the observed effects were time and concentration dependent. The most pronounced toxic effect was detected on day three when even the lowest concentration of 0.01 mg/ml led to a significant decrease in proliferation compared to control. Functionality assays of osteoblasts and skeletal muscle cells revealed a sensitive reaction of the cells to the drug, indicating that vancomycin is toxic to these cells during the process of differentiation. These data suggest that the vancomycin administration is critical for cell survival and function. Therefore, the concentration of administered antibiotics needs to be carefully evaluated to find a balance between defense against pathogens and functionality of host cells and tissues.

中文翻译：

---

万古霉素对参与组织再生的不同细胞的活力和功能的毒性作用。


为了预防感染，局部施用抗生素是一种有用的工具。特别是在骨折中，通常使用万古霉素浸渍的骨水泥，允许在感染侧使用高浓度的抗生素，而无需高血清浓度。然而，除了潜在的病原体外，参与组织再生的细胞也可能受到该药物的影响。我们研究了万古霉素对成骨细胞、内皮细胞、成纤维细胞和骨骼肌细胞的活力和功能的影响。我们的结果表明，万古霉素降低了所分析的所有细胞的活力，并且观察到的效果具有时间和浓度依赖性。在第三天检测到最明显的毒性作用，与对照相比，即使是最低浓度 0.01 mg/ml 也会导致增殖显着下降。成骨细胞和骨骼肌细胞的功能测定揭示了细胞对药物的敏感反应，表明万古霉素在分化过程中对这些细胞有毒。这些数据表明万古霉素的施用对于细胞存活和功能至关重要。因此，需要仔细评估抗生素的浓度，以找到病原体防御与宿主细胞和组织功能之间的平衡。