Low diversity intestinal dysbiosis has been associated with inflammatory bowel disease, including patients with ulcerative colitis with an ileo-anal pouch anastomosis. Furthermore, specific Escherichia coli phylogroups have been linked to inflammatory bowel disease. Our aim was to characterize the differences among microbiota and E. coli phylogroups in active and inactive pouchitis. Disease activity was assessed using the modified pouch disease activity index and by fecal calprotectin. Microbiota diversity was assessed by 16S rDNA MiSeq sequencing. E. coli phylogroup was determined after triplex PCR. Twenty patients with ulcerative colitis with an ileo-anal pouch anastomosis were included, 10 of whom had active pouchitis. Ileo-anal pouch anastomosis patients had an increased abundance of Proteobacteria colonization compared to patients with ulcerative colitis or Crohn's disease and healthy controls, p = 1.4·10-5. No differences in E. coli phylogroup colonization could be determined between cases of active and inactive disease. No significant link was found between α-diversity and pouch inflammation. However, higher levels of Fusobacteria colonization were found in patients with a pouch with a fecal calprotectin level above 500, p = 0.02. In conclusion, patients with a pouch had an increased Proteobacteria abundance, but only Fusobacteria abundance was linked to inflammation.

中文翻译：

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肠肛门袋吻合术中细菌菌的高丰度和与袋囊炎相关的融合细菌的丰度增加。

低多样性肠道功能障碍与炎症性肠病有关，包括溃疡性结肠炎伴回肠肛门袋吻合的患者。此外，特定的大肠杆菌系统群与炎症性肠病有关。我们的目的是鉴定活动性和非活动性囊炎中微生物群和大肠杆菌系统群之间的差异。使用改良的囊袋疾病活性指数和粪钙卫蛋白评估疾病活性。通过16S rDNA MiSeq测序评估微生物群多样性。三重PCR后确定大肠杆菌系统群。包括20例溃疡性结肠炎伴回肠肛门袋吻合术的患者，其中10例患有活动性囊炎。与溃疡性结肠炎或克罗恩病和健康对照组相比，肠肛门袋吻合患者的变形杆菌菌落数量增加，p = 1.4·10-5。在活动性和非活动性疾病病例之间，没有确定大肠杆菌系统群定植的差异。在α多样性和囊袋炎症之间未发现明显联系。但是，在粪便钙卫蛋白水平高于500的小袋患者中，发现的梭菌细菌定植水平更高，p = 0.02。总之，有囊袋的患者变形杆菌丰富度增加，但是只有富细菌含量才与炎症有关。在α多样性和囊袋炎症之间未发现明显联系。但是，在粪便钙卫蛋白水平高于500的小袋患者中，发现的梭菌细菌定植水平更高，p = 0.02。总之，有囊袋的患者变形杆菌丰富度增加，但只有富细菌含量才与炎症有关。在α多样性和囊袋炎症之间未发现明显联系。但是，在粪便钙卫蛋白水平高于500的小袋患者中，发现的梭菌细菌定植水平更高，p = 0.02。总之，有囊袋的患者变形杆菌丰富度增加，但只有富细菌含量才与炎症有关。