The cytokinesis-block micronucleus cytome (CBMNcyt) assay is a comprehensive method to measure DNA damage, cytostasis and cytotoxicity caused by nutritional, radiation and chemical factors. A slide imaging technique has been identified as a new method to assist with the visual scoring of cells for the CBMNcyt assay. A NanoZoomer S60 Digital Pathology slide scanner was used to view WIL2-NS cells treated with hydrogen peroxide (H₂O₂) and measure CBMNcyt assay biomarkers using a high-definition desktop computer screen. The H₂O₂-treated WIL2-NS cells were also scored visually using a standard light microscope, and the two visual scoring methods were compared. Good agreement was found between the scoring methods for all DNA damage indices (micronuclei, nucleoplasmic bridges and nuclear buds) and nuclear division index with correlation R values ranging from 0.438 to 0.789, P < 0.05. Apoptotic and necrotic cell frequency was lower for the NanoZoomer scoring method, but necrotic frequency correlated well with the direct visual microscope method (R = 0.703, P < 0.0001). Considerable advantages of the NanoZoomer scoring method compared to direct visual microscopy includes reduced scoring time, improved ergonomics and a reduction in scorer fatigue. This study indicates that a digital slide scanning and viewing technique may assist with visual scoring for the CBMNcyt assay and provides similar results to conventional direct visual scoring.

中文翻译：

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数字幻灯片扫描仪和查看技术是否可以协助对胞质阻滞性微核细胞因子测定进行视觉评分？

胞质分裂阻滞性微核细胞因子（CBMNcyt）测定法是一种综合方法，用于测量营养，放射和化学因素引起的DNA损伤，细胞停滞和细胞毒性。载玻片成像技术已被确认为一种新的方法，可帮助对CBMNcyt分析进行细胞的视觉评分。使用NanoZoomer S60数字病理幻灯片扫描仪查看用过氧化氢（H ₂ O ₂）处理的WIL2-NS细胞，并使用高清台式计算机屏幕测量CBMNcyt分析生物标记。H ₂ O ₂还使用标准光学显微镜对经处理的WIL2-NS细胞进行了视觉评分，并比较了两种视觉评分方法。在所有DNA损伤指数（微核，核质桥和核芽）的评分方法与核分裂指数之间具有良好的一致性，相关R值在0.438至0.789之间，P <0.05。NanoZoomer评分方法的凋亡和坏死细胞频率较低，但坏死频率与直接视觉显微镜法相关性很好（R = 0.703，P<0.0001）。与直接视觉显微镜相比，NanoZoomer评分方法的显着优势包括减少评分时间，改善人体工程学和减少刻痕疲劳。这项研究表明，数字幻灯片扫描和查看技术可能有助于CBMNcyt分析的视觉评分，并提供与常规直接视觉评分相似的结果。