Oral consumption of probiotics is practical and can be an effective solution to preserve vaginal eubiosis. Here, we studied the ability of orally administered Lactobacillus paracasei LPC-S01 (DSM26760) to affect the composition of the vaginal microbiota and colonize the vaginal mucosa in nondiseased adult women. Forty volunteers took oral probiotic (24 billion CFU) or placebo capsules daily for four weeks, and after a four-week washout, they switched to placebo or probiotic capsules according to the crossover design. Twenty-three volunteers completed the study according to the protocol. Before and after capsule ingestion, vaginal swabs were collected for qPCR quantification to detect L. paracasei LPC-S01 and for 16S rRNA gene sequencing. Vaginal swabs were grouped according to their bacterial taxonomic structure into nine community state types (CSTs), four of which were dominated by lactobacilli. L. paracasei LPC-S01 was detected in the vagina of two participants. Statistical modeling (including linear mixed-effects model analysis) demonstrated that daily intake of probiotic capsules reduced the relative abundance of Gardnerella spp. Quantitative PCR with G. vaginalis primers confirmed this result. Considering the pathogenic nature of Gardnerella vaginalis, these results suggest a potential positive effect of this probiotic capsule on the vaginal microbial ecosystem.

中文翻译：

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口服含副干酪乳杆菌LPC-S01的胶囊对健康成年女性阴道菌群的影响：一项随机，安慰剂对照，双盲交叉研究。

口服益生菌是切实可行的，可以作为保持阴道优生的有效解决方案。在这里，我们研究了口服口服副干酪乳杆菌LPC-S01（DSM26760）影响阴道微生物群组成并在未患病的成年女性中定殖阴道粘膜的能力。四十名志愿者每天服用口服益生菌（240亿个CFU）或安慰剂胶囊，持续四个星期，冲洗四周后，根据交叉设计，他们改用安慰剂或益生菌胶囊。根据协议，有23名志愿者完成了这项研究。摄取胶囊之前和之后，收集阴道拭子用于qPCR定量以检测副干酪乳杆菌LPC-S01和16S rRNA基因测序。阴道拭子根据其细菌分类结构分为九种社区状态类型（CST），其中四种以乳杆菌为主。在两名参与者的阴道中检测到副干酪乳杆菌LPC-S01。统计模型（包括线性混合效应模型分析）表明，益生菌胶囊的每日摄入量减少了加德纳菌的相对丰度。用阴道加德纳氏菌引物定量PCR证实了该结果。考虑到阴道加德纳氏菌的致病性，这些结果表明该益生菌胶囊对阴道微生物生态系统具有潜在的积极作用。统计模型（包括线性混合效应模型分析）表明，益生菌胶囊的每日摄入量减少了加德纳菌的相对丰度。用阴道加德纳氏菌引物定量PCR证实了该结果。考虑到阴道加德纳菌的致病性，这些结果表明该益生菌胶囊对阴道微生物生态系统具有潜在的积极作用。统计模型（包括线性混合效应模型分析）表明，益生菌胶囊的每日摄入量减少了加德纳菌的相对丰度。用阴道加德纳氏菌引物定量PCR证实了该结果。考虑到阴道加德纳菌的致病性，这些结果表明该益生菌胶囊对阴道微生物生态系统具有潜在的积极作用。