Carotenoid cleavage products (CCPs) can serve critical functions including hormones, volatiles, signals, and growth inhibitors. However, little is known about the regulation of carotenoid degradation and function of CCPs in high carotenoid-accumulating Dunaliella species. In this study, high salt stress resulted in high accumulation of CCPs including α-ionone, β-ionone and β-cyclocitral in Dunaliella sp. FACHB-847. Under salt stress, carotenoid degradation of Dunaliella sp. was dependent of the expression levels of carotenoid cleavage dioxygenase genes to some extent and may occur mostly non-enzymatically. Exposure of Dunaliella cells to exogenous β-ionone and β-cyclocitral (10 mg/L) induced the generation of reactive oxygen species, enhanced malondialdehyde levels, decreased cell growth and chlorophylls, and down-regulated genes involved in carbon metabolism, biosynthesis of amino acid, and energy metabolism related to cell development and growth. β-ionone and β-cyclocitral also repressed carbon fixation and chlorophyll biosynthesis, which was in agreement with the decreased chlorophylls. β-ionone up-regulated genes involved in the biosynthesis of cellular membrane due to the severe cell damage. β-cyclocitral down-regulated genes responsible for photosynthesis, cell growth and stress response, which may lead to the imbalance of redox homeostasis and cell damage. Taken together, sufficient levels of β-ionone and β-cyclocitral can act as growth inhibitors suppressing the growth of Dunaliella cells.

类胡萝卜素裂解产物（CCP）可以发挥关键功能，包括激素，挥发物，信号和生长抑制剂。但是，关于高类胡萝卜素积累杜氏藻中类胡萝卜素降解和CCP功能的调控知之甚少。在这项研究中，高盐胁迫导致杜氏藻（Dunaliella sp。）中包括α-紫罗兰酮，β-紫罗兰酮和β-环柠檬醛在内的CCP大量积累。FACHB-847。在盐胁迫下，杜氏藻的类胡萝卜素降解。在某种程度上取决于类胡萝卜素裂解双加氧酶基因的表达水平，并且可能主要是非酶促发生的。杜氏藻的暴露外源β-紫罗兰酮和β-环柠檬酸（10 mg / L）的细胞诱导活性氧的生成，丙二醛水平提高，细胞生长和叶绿素下降，碳代谢，氨基酸生物合成和相关基因的下调与细胞发育和成长有关的能量代谢。β-紫罗兰酮和β-环柠檬醛也抑制了碳固定和叶绿素的生物合成，这与减少的叶绿素一致。由于严重的细胞损伤，β-紫罗兰酮上调了参与细胞膜生物合成的基因。β-环柠檬酸下调的基因负责光合作用，细胞生长和应激反应，可能导致氧化还原稳态和细胞损伤的失衡。在一起杜氏藻细胞。