The genus Minibiotus is morphologically diverse, which may suggest its polyphyletic character. However, scarce genetic data and often also the lack of detailed morphological data currently do not allow for the verification of the relationships within this genus. Here, for the very first time, we provide an integrative description of a new Minibiotus species. Minibiotus ioculator sp. nov. from the Republic of South Africa differs from other congeners mainly by egg ornamentation with processes on the egg shell that resemble the hat of a royal jester. We also provide new taxonomic data on Minibiotus pentannulatus based on a population newly found in Tanzania, which constitutes the first African record of this species originally described from South America. Our study involved both classical taxonomic methods, which include morphological and morphometric analyses conducted with the use of light and scanning electron microscopy, and genetic data in the form of DNA sequences of four markers (three nuclear: 18S rRNA, 28S rRNA, ITS-2, and one mitochondrial: COI). The results of this study allow a discussion of species composition within Minibiotus and question the validity of the current diagnosis of the genus.

属Minibiotus是形态差异较大，这可能意味着它的多系特征。但是，目前缺乏遗传数据，而且通常也缺乏详细的形态学数据，无法验证该属内的关系。在这里，我们首次提供了一种新的迷你bio属物种的综合描述。Minibiotus接种器 sp。十一月 来自南非共和国的产品与其他同类产品的不同之处主要在于蛋的装饰，蛋壳上的装饰过程类似于皇室小丑的帽子。我们还提供了有关五角Mini小生物的新分类学数据。基于在坦桑尼亚新发现的种群，这是最初从南美描述的该物种的第一个非洲记录。我们的研究涉及两种经典的分类学方法，包括使用光镜和扫描电镜进行形态学和形态计量学分析，以及以四种标记物（三核：18S rRNA，28S rRNA，ITS-2）的DNA序列形式的遗传数据。 ，以及一个线粒体：COI）。这项研究的结果允许讨论Minibiotus内的物种组成，并质疑当前属诊断的有效性。