Comparative analysis using complete ITS2 and partial 28S rDNA sequence data revealed that cercariaeum developing in rediae inLithoglyphus naticoidesrepresent two different lissorchiid species. One morphotype of cercariaeum is conspecific with adultPalaeorchis incognitusfrom European roach,Rutilus rutilus. The other cercariaeum is attributable to the genusAsymphylodora, but the species identity is not yet determined. We also generate the first rDNA sequences forAsymphylodora progeneticabased on new collections fromBithynia tentaculatafrom Lithuania. Phylogenetic analyses of the newly generated sequences, together with information for other lissorchiids available on GenBank, showed that all representatives of Lissorchiidae form a strongly supported clade. Three monophyletic lineages,Asymphylodora,PalaeorchisandLissorchis, were recognized at the generic level. Karyological analysis of the chromosome set of larvalP. incognitusrevealed a diploid number of 2n= 20. Its karyotype with subtelocentric chromosomes prevailing can be regarded as comparatively ‘primitive’, which is consistent with the basal position ofP. incognitusin the 28S tree relative to the representatives of the genusAsymphylodora. The present study adds significant new information for establishing species-specific markers for the confident characterization of different developmental stages of lissorchiid species and clarification of their life cycles.

中文翻译：

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探索欧洲淡水中与砾石蜗牛 Lithoglyphus naticoides 相关的 lissorchiid 吸虫 (Digenea: Lissorchiidae) 的物种多样性

使用完整 ITS2 和部分 28S rDNA 序列数据的比较分析表明，尾蚴在石纹海藻代表两种不同的 lissorchiid 物种。尾蚴的一种形态型与成虫同种无名古兰来自欧洲蟑螂，金红花. 其他尾蚴归属于该属空心莲，但物种身份尚未确定。我们还生成了第一个 rDNA 序列遗传病菌基于来自的新系列触须草来自立陶宛。对新产生的序列的系统发育分析，连同 GenBank 上可用的其他 lissorchiids 的信息，表明 Lissorchiidae 的所有代表形成了一个强烈支持的进化枝。三个单系谱系，空心莲,古兰科和利索基斯，在通用级别上得到认可。幼虫染色体组的染色体组学分析P. incognitus显示二倍体数为 2n= 20. 以近端着丝粒染色体为主的核型可以认为是比较“原始”的，这与P. incognitus在相对于该属的代表的28S树中空心莲. 本研究为建立物种特异性标记物增加了重要的新信息，以可靠地表征 lissorchiid 物种的不同发育阶段并阐明其生命周期。